In:
Nanoscience and Nanotechnology Letters, American Scientific Publishers, Vol. 12, No. 4 ( 2020-04-01), p. 525-535
Abstract:
MicroRNA-155 plays an important role in the pathogenesis, progression, and treatment of various cancers. It is abnormally expressed in gastric cancer, but its expression level, function, and significance remain controversial. Therefore, we studied these facets of microRNA-155 in gastric
cancer. A total of 700 (miRDB) and 556 (TargetScan) target genes were obtained and 280 genes were identified by Venn mapping, 49 of which had a target score of 90 or higher. GO and KEGG analysis revealed that they were associated with the autophagy and apoptosis pathways. Rictor and Fos were selected for further study. Thirty-two cases exhibited high microRNA-155 expression (group H) and 11 cases showed low expression (group L). Twelve patients had high Rictor expression, whereas 31 patients exhibited low expression. Thirteen cases showed normal Fos expression and 30 cases had
low or negative expression. Thirty-three cases had high Beclin1/LC3 expression, whereas 10 cases exhibited low expression. Ten cases had high caspase-3 and caspase-9 expression, whereas 33 cases had low expression. According to the results of immunohistochemistry and w estern blot analyses,
Rictor, Fos, caspase-3, and caspase-9 were minimally expressed, whereas Beclin1 and LC3 were highly expressed in group H. However, all six genes exhibited no significant expression differences in group L. The abnormal expression of microRNA-155 may indicate the occurrence of gastric cancer and its expression level was negatively correlated with the clinical stage of cancer. Down-regulated expression of Rictor and Fos, enhancement of autophagy, and reduced apoptosis may be related to the overexpression of microRNA-155. MicroRNA-155 may promote the progression of gastric cancer
by promoting autophagy and inhibiting apoptosis.
Type of Medium:
Online Resource
ISSN:
1941-4900
DOI:
10.1166/nnl.2020.3144
Language:
English
Publisher:
American Scientific Publishers
Publication Date:
2020
Permalink
