In:
The Journal of Immunology, The American Association of Immunologists, Vol. 120, No. 5 ( 1978-05-01), p. 1760-1760
Abstract:
Much interest was generated when the C3H/HeJ mouse was considered to be refractory to endotoxin, a biologically active product released from Gram-negative bacteria (1, 2). This interest was partly because such a mouse would permit a simple, indirect assay for possible endotoxin contamination in biologic preparations exhibiting the endotoxin-like activity of B cell mitogenesis. For example, if C3H/HeJ splenic B cells with a presumed inability to respond to endotoxin proliferated upon exposure to a certain substance, the mitogenic effect would presumably be due to that substance, not to endotoxin contamination. A precedent for use of the C3H/HeJ mouse in this manner was set with purified protein derivitive (PPD) (1). It was shown that PPD caused proliferation of splenic mouse B cells, including those of the C3H/HeJ to levels equaling that of other mice. Since the C3H/HeJ was considered a low responder to endotoxin, endotoxin contaminants in PPD were excluded as the source of B cell mitogenicity.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.120.5.1760
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1978
detail.hit.zdb_id:
1475085-5
detail.hit.zdb_id:
3056-9
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