In:
European Journal of Immunology, Wiley, Vol. 25, No. 5 ( 1995-05), p. 1399-1404
Abstract:
LEG rats are known to show a maturational arrest in the development of CD4 + 8 + to CD4 + 8 − cells in the thymus. Despite the blockade of maturation of CD4 + 8 − thymocytes, CD4 + T cells were observed in peripheral lymphoid organs, and these cells exhibit a defect in interleukin‐2 (IL‐2) production upon concanavalin A (Con A) stimulation. Although peripheral CD4 + cells in normal rat highly expressed CD45RC (CD45RC high ), the level of CD45RC expression was low (CD45RC low ) in LEC rat peripheral CD4 + cells. However, CD4 + cells from both strains highly expressed CD45 when those cells were stained by pan‐CD45 mAb, suggesting that LEC rat CD4 + cells are deficient in expression of the CD45RC isoform, but not of CD45 molecules. When backcross rats from (F344 × LEC)F 1 × LEC were examined, the phenotype for CD45 expression pattern in CD4 + cells was clearly correlated with IL‐2 production level in response to Con A stimulation. Thus, CD45RC low cells exhibit a defect in IL‐2 production, while CD45RC high cells show normal IL‐2 production. Protein tyrosine phosphatase (PTPase) activity in the membrane fraction of LEC rat CD4 + cells was threefold higher than that of normal rat CD4 + cells. Con A stimulation led to an increase in tyrosine phosphorylation levels, especially 100‐ and 40‐kDa proteins, in normal rat CD4 + cells. In LEC rat CD4 + cells, however, the level of tyrosine phosphorylation in those proteins were very low. These results suggest that an elevated CD45 PTPase activity is responsive for a defect in IL‐2 production in LEC rat peripheral CD4 + T cells.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.1830250539
Language:
English
Publisher:
Wiley
Publication Date:
1995
detail.hit.zdb_id:
1491907-2
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