In:
Nephron Experimental Nephrology, S. Karger AG, Vol. 111, No. 1 ( 2008-12-2), p. e20-e30
Abstract:
〈 i 〉 Backgrounds/Aims: 〈 /i 〉 It has been reported that urinary oxidative stress markers are higher in diabetic patients with proteinuria. We performed the present study to elucidate the relationship between urinary excretion of oxidative stress markers, albumin excretion, and histological changes, and to confirm the potential utility of oxidative stress markers for clinical treatment. 〈 i 〉 Methods: 〈 /i 〉 Diabetic 〈 i 〉 db/db 〈 /i 〉 mice or nondiabetic 〈 i 〉 db/m 〈 /i 〉 mice were administered candesartan (10 mg/kg/day) or hydralazine (50 mg/kg/day) for 18 weeks. 〈 i 〉 Results: 〈 /i 〉 Thirty-week-old male 〈 i 〉 db/db 〈 /i 〉 mice treated with control vehicle revealed elevated urinary excretion and immunohistological levels of 8-hydroxydeoxyguanosine in glomeruli when compared to 〈 i 〉 db/m 〈 /i 〉 mice. Treatment with candesartan, but not hydralazine, reduced these values to levels in 〈 i 〉 db/m 〈 /i 〉 mice. Increased mesangial expansion, urinary excretion of albumin and 8-isoprostane, and glomerular immunohistological levels of nitrotyrosine in 〈 i 〉 db/db 〈 /i 〉 mice were also decreased markedly by candesartan but not hydralazine. Interestingly, correlations between levels of albumin and oxidative stress markers in urine were very high, even when groups undergoing long-term (44 weeks) treatment were included (correlation coefficient 0.767 with respect to 8-hydroxydeoxyguanosine, 0.888 with respect to 8-isoprostane). 〈 i 〉 Conclusion: 〈 /i 〉 It is anticipated that urinary concentrations of oxidative stress markers will be direct barometers of glomerulus-derived oxidative stress and glomerular injury in diabetic nephropathy.
Type of Medium:
Online Resource
ISSN:
1660-2129
Language:
English
Publisher:
S. Karger AG
Publication Date:
2008
detail.hit.zdb_id:
2098337-2
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