In:
Rapid Communications in Mass Spectrometry, Wiley, Vol. 16, No. 6 ( 2002-03-30), p. 531-536
Abstract:
A one‐step phosphoryl derivatization method has been used in a peptide sequencing procedure for electrospray ionization tandem mass spectrometry (ESI‐MS/MS). The sodiated derivatized peptides exhibit very simple dissociation patterns, in which two kinds of fragment ions, [b n + OH + Na] + and [a n + Na] + , are formed. Since the amino acid residues are lost sequentially from the C‐terminus, peptide sequences can be identified easily. The fragmentation efficiency of peptides increased as a result of the phosphorylation, and also provided peaks of useful intensity at lower m/z . A peptide with lysine at the C‐terminus was derivatized and analyzed by ESI‐MS/MS. Similar mass spectra, from which the sequence could be read out, were obtained. This is a novel derivatization method yielding neutral derivatives that should be suitable for peptide sequencing by LC/ESI‐MS/MS. Copyright © 2002 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0951-4198
,
1097-0231
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
2002158-6
detail.hit.zdb_id:
58731-X
SSG:
11
Permalink