In:
Journal of Separation Science, Wiley, Vol. 36, No. 23 ( 2013-12), p. 3709-3716
Abstract:
Ginger, a widely used spice and traditional C hinese medicine, is prone to be contaminated by mycotoxins. A simple, sensitive, and reproducible method based on immunoaffinity column clean‐up coupled with HPLC and on‐line postcolumn photochemical derivatization with fluorescence detection was developed for the simultaneous determination of aflatoxins ( AF s) B 1 , B 2 , G 1 , G 2 , and ochratoxin A ( OTA ) in 25 batches of gingers and related products marketed in C hina for the first time. The samples were first extracted by ultrasonication with methanol/water (80:20, v/v) and then cleaned up with immunoaffinity columns for analysis. Under the optimized conditions, the LOD s and LOQ s for the five mycotoxins were 0.03–0.3 and 0.1–0.9 μg/kg, respectively. The average recoveries ranged from 81.3–100.8% for AF s and from 88.6–99.5% for OTA at three spiking levels. Good linearity was observed for the analytes with correlation coefficients all 〉 0.9995. All moldy gingers were contaminated with at least one kind of the five investigated mycotoxins, while none of them were found in normal gingers. Ginger powder samples were contaminated slightly with the contamination levels below the LOQ s, while ginger tea bags were mainly contaminated by OTA at 1.05–1.19 μg/kg and ginger black tea bags were mainly contaminated by AF s at 3.37–5.76 μg/kg. All the contamination levels were below the legally allowable limits.
Type of Medium:
Online Resource
ISSN:
1615-9306
,
1615-9314
DOI:
10.1002/jssc.201300885
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2047990-6
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