In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2022-2-10), p. e0010172-
Abstract:
Burkholderia pseudomallei is a soil-dwelling bacterium endemic to Southeast Asia and northern Australia that causes the disease, melioidosis. Although the global genomic diversity of clinical B . pseudomallei isolates has been investigated, there is limited understanding of its genomic diversity across small geographic scales, especially in soil. In this study, we obtained 288 B . pseudomallei isolates from a single soil sample (~100g; intensive site 2, INT2) collected at a depth of 30cm from a site in Ubon Ratchathani Province, Thailand. We sequenced the genomes of 169 of these isolates that represent 7 distinct sequence types (STs), including a new ST (ST1820), based on multi-locus sequence typing (MLST) analysis. A core genome SNP phylogeny demonstrated that all identified STs share a recent common ancestor that diverged an estimated 796–1260 years ago. A pan-genomics analysis demonstrated recombination between clades and intra-MLST phylogenetic and gene differences. To identify potential differential virulence between STs, groups of BALB/c mice (5 mice/isolate) were challenged via subcutaneous injection (500 CFUs) with 30 INT2 isolates representing 5 different STs; over the 21-day experiment, eight isolates killed all mice, 2 isolates killed an intermediate number of mice (1–2), and 20 isolates killed no mice. Although the virulence results were largely stratified by ST, one virulent isolate and six attenuated isolates were from the same ST (ST1005), suggesting that variably conserved genomic regions may contribute to virulence. Genomes from the animal-challenged isolates were subjected to a bacterial genome-wide association study to identify genomic regions associated with differential virulence. One associated region is a unique variant of Hcp1, a component of the type VI secretion system, which may result in attenuation. The results of this study have implications for comprehensive sampling strategies, environmental exposure risk assessment, and understanding recombination and differential virulence in B . pseudomallei .
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010172
DOI:
10.1371/journal.pntd.0010172.g001
DOI:
10.1371/journal.pntd.0010172.g002
DOI:
10.1371/journal.pntd.0010172.g003
DOI:
10.1371/journal.pntd.0010172.g004
DOI:
10.1371/journal.pntd.0010172.g005
DOI:
10.1371/journal.pntd.0010172.t001
DOI:
10.1371/journal.pntd.0010172.s001
DOI:
10.1371/journal.pntd.0010172.s002
DOI:
10.1371/journal.pntd.0010172.s003
DOI:
10.1371/journal.pntd.0010172.s004
DOI:
10.1371/journal.pntd.0010172.s005
DOI:
10.1371/journal.pntd.0010172.s006
DOI:
10.1371/journal.pntd.0010172.s007
DOI:
10.1371/journal.pntd.0010172.s008
DOI:
10.1371/journal.pntd.0010172.s009
DOI:
10.1371/journal.pntd.0010172.s010
DOI:
10.1371/journal.pntd.0010172.s011
DOI:
10.1371/journal.pntd.0010172.s012
DOI:
10.1371/journal.pntd.0010172.s013
DOI:
10.1371/journal.pntd.0010172.s014
DOI:
10.1371/journal.pntd.0010172.r001
DOI:
10.1371/journal.pntd.0010172.r002
DOI:
10.1371/journal.pntd.0010172.r003
DOI:
10.1371/journal.pntd.0010172.r004
DOI:
10.1371/journal.pntd.0010172.r005
DOI:
10.1371/journal.pntd.0010172.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5
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