In:
Acta Endocrinologica, Oxford University Press (OUP), Vol. 128, No. 4 ( 1993-04), p. 361-366
Abstract:
Epidermal growth factor (EGF) stimulated the prolactin (PRL) synthesis and release from the GH 4 C 1 cells in a dose-dependent manner. The ED 50 was between 10 −11 and 10 −10 mol/l. The maximal effect was obtained at 10 −9 mol/l EGF for the release, and 10 −8 mol/l EGF for the synthesis. EGF stimulated the release of PRL from cell perfusion columns after a lag period of about 30 s. The maximal secretion of PRL occurred about 60 s after the start of stimulation. The PRL secretion declined to basal levels within 2 min. The EGF-stimulated PRL release was additive to the secretion evoked by thyrotropin-releasing hormone (TRH) and vasoactive intestinal peptide (VIP). An instantaneous increase in the intracellular concentration of free calcium, [Ca 2+ ] i , of the GH 4 C 1 cells was observed after the administration of EGF. EGF modified neither the basal nor the TRH-stimulated inositoltrisphosphate production in the GH 4 C 1 cells, and EGF did not show any effect on the cyclic adenosine monophosphate production of these cells.
Type of Medium:
Online Resource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/acta.0.1280361
Language:
Unknown
Publisher:
Oxford University Press (OUP)
Publication Date:
1993
detail.hit.zdb_id:
1485160-X
Permalink