GLORIA — GEOMAR Library Ocean Research Information Access

Hits per page

hits 1 - 4 | 4 hits

Sorting

Online Resource

Bio 3D Conduits Derived from Bone Marrow Stromal Cells Promote Peripheral Nerve Regeneration

Yurie, Hirofumi ; Ikeguchi, Ryosuke ; Aoyama, Tomoki ; [et al.]

SAGE Publications ; 2020

In: Cell Transplantation Vol. 29 ( 2020-01-01), p. 096368972095155-

add to mindlist on the mindlist

Details

In: Cell Transplantation, SAGE Publications, Vol. 29 ( 2020-01-01), p. 096368972095155-

Abstract: We previously reported that a nerve conduit created from fibroblasts promotes nerve regeneration in a rat sciatic nerve model. This study aims to determine whether a nerve conduit created from bone marrow stromal cells (BMSCs) can promote nerve regeneration. Primary BMSCs were isolated from femur bone marrow of two Lewis rats, and cells at passages 4–7 were used. We created seven Bio 3D nerve conduits from BMSCs using a Bio-3D Printer. The conduits were transplanted to other Lewis rats to bridge 5-mm right sciatic nerve gaps (Bio 3D group, n = 7). We created two control groups: a silicone group (S group, n = 5) in which the same nerve gap was bridged with a silicone tube, and a silicone cell group (SC group, n = 5) in which the gap was bridged with a BMSC injection. Twelve weeks after transplantation, nerve regeneration was evaluated functionally and morphologically. In addition, PKH26-labeled BMSCs were used to fabricate a Bio 3D conduit that was transplanted for cell trafficking analysis. Electrophysiological study, kinematic analysis, wet muscle weight, and morphological parameters showed significantly better nerve regeneration in the Bio 3D group than in the S group or SC group. In immunohistochemical studies, sections from the Bio 3D group contained abundant S-100-positive cells. In cell trafficking analysis, PKH26-positive cells stained positive for the Schwann cell markers S-100, p75NTR, and GFAP. Bio 3D nerve conduits created from BMSCs can promote peripheral nerve regeneration in a rat sciatic nerve model through BMSC differentiation into Schwann-like cells.

Type of Medium: Online Resource

ISSN: 0963-6897 , 1555-3892

URL: Article

DOI: 10.1177/0963689720951551

Language: English

Publisher: SAGE Publications

Publication Date: 2020

detail.hit.zdb_id: 2020466-8

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

Online Resource

Inhibition of RANKL Expression in Osteocyte-like Differentiated Tumor Cells in Giant Cell Tumor of Bone After Denosumab Treatment

Noguchi, Takashi ; Sakamoto, Akio ; Murotani, Yoshiki ; [et al.]

SAGE Publications ; 2023

In: Journal of Histochemistry & Cytochemistry Vol. 71, No. 3 ( 2023-03), p. 131-138

add to mindlist on the mindlist

Details

In: Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 71, No. 3 ( 2023-03), p. 131-138

Abstract: Giant cell tumors of bone (GCTBs) are locally aggressive tumors with the histological features of giant cells and stromal cells. Denosumab is a human monoclonal antibody that binds to the cytokine receptor activator of nuclear factor–kappa B ligand (RANKL). RANKL inhibition blocks tumor-induced osteoclastogenesis, and survival, and is used to treat unresectable GCTBs. Denosumab treatment induces osteogenic differentiation of GCTB cells. In this study, the expression of RANKL, special AT-rich sequence-binding protein 2 (SATB2, a marker of osteoblast differentiation), and sclerostin/SOST (a marker of mature osteocytes) was analyzed before and after treatment with denosumab in six cases of GCTB. Denosumab therapy was administered a mean of five times over a mean 93.5-day period. Before denosumab treatment, RANKL expression was observed in one of six cases. After denosumab therapy, spindle-like cells devoid of giant cell aggregation were RANKL-positive in four of six cases. Bone matrix–embedded osteocyte markers were observed, although RANKL was not expressed. Osteocyte-like cells were confirmed to have mutations, as identified using mutation-specific antibodies. Our study results suggest that treatment of GCTBs with denosumab results in osteoblast–osteocyte differentiation. Denosumab played a role in the suppression of tumor activity via inhibition of the RANK–RANKL pathway, which triggers osteoclast precursors to differentiate into osteoclasts.

Type of Medium: Online Resource

ISSN: 0022-1554 , 1551-5044

URL: Article

DOI: 10.1369/00221554231163638

Language: English

Publisher: SAGE Publications

Publication Date: 2023

detail.hit.zdb_id: 1421306-0

SSG: 12

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

Online Resource

Long-Term Outcome of Sciatic Nerve Regeneration Using Bio3D Conduit Fabricated from Human Fibroblasts in a Rat Sciatic Nerve Model

Ando, Maki ; Ikeguchi, Ryosuke ; Aoyama, Tomoki ; [et al.]

SAGE Publications ; 2021

In: Cell Transplantation Vol. 30 ( 2021-01-01), p. 096368972110213-

add to mindlist on the mindlist

Details

In: Cell Transplantation, SAGE Publications, Vol. 30 ( 2021-01-01), p. 096368972110213-

Abstract: Previously, we developed a Bio3D conduit fabricated from human fibroblasts and reported a significantly better outcome compared with artificial nerve conduit in the treatment of rat sciatic nerve defect. The purpose of this study is to investigate the long-term safety and nerve regeneration of Bio3D conduit compared with treatments using artificial nerve conduit and autologous nerve transplantation. We used 15 immunodeficient rats and randomly divided them into three groups treated with Bio3D ( n = 5) conduit, silicon tube ( n = 5), and autologous nerve transplantation ( n = 5). We developed Bio3D conduits composed of human fibroblasts and bridged the 5 mm nerve gap created in the rat sciatic nerve. The same procedures were performed to bridge the 5 mm gap with a silicon tube. In the autologous nerve group, we removed the 5 mm sciatic nerve segment and transplanted it. We evaluated the nerve regeneration 24 weeks after surgery. Toe dragging was significantly better in the Bio3D group (0.20 ± 0.28) than in the silicon group (0.6 ± 0.24). The wet muscle weight ratios of the tibial anterior muscle of the Bio3D group (79.85% ± 5.47%) and the autologous nerve group (81.74% ± 2.83%) were significantly higher than that of the silicon group (66.99% ± 3.51%). The number of myelinated axons and mean myelinated axon diameter was significantly higher in the Bio3D group (14708 ± 302 and 5.52 ± 0.44 μm) and the autologous nerve group (14927 ± 5089 and 6.04 ± 0.85 μm) than the silicon group (7429 ± 1465 and 4.36 ± 0.21 μm). No tumors were observed in any of the rats in the Bio3D group at 24 weeks after surgery. The Bio3D group showed significantly better nerve regeneration and there was no significant difference between the Bio3D group and the nerve autograft group in all endpoints.

Type of Medium: Online Resource

ISSN: 0963-6897 , 1555-3892

URL: Article

DOI: 10.1177/09636897211021357

Language: English

Publisher: SAGE Publications

Publication Date: 2021

detail.hit.zdb_id: 2020466-8

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

Online Resource

Clinical Results of Arthroscopic Partial Trapeziectomy With Suture-Button Suspensionplasty for Thumb Carpometacarpal Arthritis

Maeda, Akemi ; Ikeguchi, Ryosuke ; Noguchi, Takashi ; [et al.]

SAGE Publications ; 2023

In: HAND Vol. 18, No. 5 ( 2023-07), p. 740-745

add to mindlist on the mindlist

Details

In: HAND, SAGE Publications, Vol. 18, No. 5 ( 2023-07), p. 740-745

Abstract: This study represents the clinical results, especially range of motion (ROM) improvement, of arthroscopic partial trapeziectomy with suture-button suspensionplasty for symptomatic grade II and III thumb carpometacarpal arthritis with a minimum 1-year follow-up. Methods: Thirty-two patients (mean: 67.5 years) with grade II and III thumb carpometacarpal arthritis treated with arthroscopic partial trapeziectomy with suture-button suspensionplasty were retrospectively followed up for at least 1 year. The physical assessments included ROM, pain visual analogue scale (VAS), strength, and the Disabilities of the Arm, Shoulder, and Hand (DASH) questionnaire. The physical variables were retrospectively compared before surgery and at the final follow-up. Results: Preoperative radial abduction and palmar abduction (45.4 ± 16.4° and 54.3 ± 13.9°, respectively) were significantly increased at the final follow-up (59.7 ± 16.9° and 65.5 ± 14.2°, respectively). Preoperative VAS score, pinch strength, and DASH score (70.5 ± 14.0, 57.2 ± 24.8% and 36.8 ± 14.8, respectively) were also significantly improved at the final follow-up (7.9 ± 9.1, 91.0 ± 39.6%, and 11.7 ± 10.5, respectively). Complications involved 1 case of irritation of the superficial branch of the radial nerve and 1 case of dystonia. Two suture-buttons were removed due to patient discomfort. Conclusions: A significant increase in ROM and pain relief was obtained after suture-button suspensionplasty with arthroscopic partial trapeziectomy.

Type of Medium: Online Resource

ISSN: 1558-9447 , 1558-9455

URL: Article

DOI: 10.1177/15589447221075663

Language: English

Publisher: SAGE Publications

Publication Date: 2023

detail.hit.zdb_id: 2316440-2

Permalink

	Location	Call Number	Limitation	Availability

Others were also interested in ...

Online Resource

Link to publisher

hits 1 - 4 | 4 hits