In:
Frontiers in Physiology, Frontiers Media SA, Vol. 12 ( 2021-7-2)
Abstract:
DNA methylation is a key epigenetic mechanism involved in embryonic muscle development and plays an important role in early muscle development. In this study, we sought to investigate the effects of genome-wide DNA methylation by combining the expression profiles of the chicken embryonic muscle. Genome-wide DNA methylation maps and transcriptomes of muscle tissues collected from different embryonic development points (E7, E11, E17, and D1) were used for whole-genome bisulfite sequencing (WGBS) and RNA sequencing, respectively. We found that the differentially methylated genes (DMGs) were significantly associated with muscle organ development, regulation of skeletal muscle satellite cell proliferation, and actin filament depolymerization. Furthermore, genes TBX1 , MEF2D , SPEG , CFL2 , and TWF2 were strongly correlated with the methylation-caused expression switch. Therefore, we chose the CFL2 gene to explore its function in skeletal muscle satellite cells, and the in vitro experiments showed that CFL2 acts as a negative regulator of chicken skeletal muscle satellite cell proliferation and can induce cell apoptosis. These results provide valuable data for future genome and epigenome studies of chicken skeletal muscle and may help reveal the molecular mechanisms of potential economic traits.
Type of Medium:
Online Resource
ISSN:
1664-042X
DOI:
10.3389/fphys.2021.697121
DOI:
10.3389/fphys.2021.697121.s001
DOI:
10.3389/fphys.2021.697121.s002
DOI:
10.3389/fphys.2021.697121.s003
DOI:
10.3389/fphys.2021.697121.s004
DOI:
10.3389/fphys.2021.697121.s005
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2564217-0
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