In:
The Journal of Immunology, The American Association of Immunologists, Vol. 184, No. 1_Supplement ( 2010-04-01), p. 132.34-132.34
Abstract:
We have found two subsets of human CD8+ T cells expressing IL-7Rαhigh and IL-7Rαlow with different cell survival responses to IL-7. These IL-7Rαlow memory CD8+ T cells that produce effector cytokines and perforin have impaired survival in response to IL-7. Although these CD8+ T cell subsets fail to respond to TCR triggering, the mechanism for this is unknown. Thus we investigated the intracellular signaling pathways in differentially regulating proliferation and effector cytokine production in human IL-7Rαlow memory CD8+ T cells. Treating these CD8+ T cells with anti-CD3 Abs did not produce IL-2, but expressed IFN-γ and TNF-α. Although IL-7Rαlow memory CD8+ T cells showed anergic phenotypes in terms of cytokine production and proliferation, activation of MAPK pathway, which is known defects in anergic T cells, was intact. Importantly, human IL-7Rαlow memory CD8+ T cells had reduced calcium flux upon in vitro TCR stimulation, leading to impaired IL-2 production. Moreover, inhibition of IL-2 production was not recovered by PMA/ionomycin treatment. There findings provide new sight into that human IL-7Rαlow memory CD8+ T cells are rendered cellular characteristics of anergic with calcium signaling defects.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.184.Supp.132.34
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2010
detail.hit.zdb_id:
1475085-5
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