In:
FEBS Letters, Wiley, Vol. 481, No. 1 ( 2000-09-08), p. 68-72
Abstract:
Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase yields (all‐ E )‐C 20 prenyl diphosphate as a final product. The three‐dimensional model of the enzyme suggested that removing two bulky residues at 77 and 114 would allow additional prenyl‐chain elongation. To test this, we examined several mutants with substitutions at 77 and/or 114. As a result, the mutants, F77G, F77G and H114A, F77G and H114G, H114A, and H114G gave C 30 , C 45 , C 50 , C 30 and C 40 as the main long product, respectively. These observations indicate that histidine‐114 plays a crucial role in chain‐length determination along with phenylalanine‐77.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(00)01972-4
Language:
English
Publisher:
Wiley
Publication Date:
2000
detail.hit.zdb_id:
1460391-3
SSG:
12
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