In:
Letters in Drug Design & Discovery, Bentham Science Publishers Ltd., Vol. 16, No. 5 ( 2019-04-15), p. 502-511
Abstract:
The emergence of fungal resistance to commercial drugs has been observed,
and because of that, research with natural products have been performed with the aim of obtaining bioactive compounds. Objective: Evaluate the chemical composition and antifungal activity of the ethanolic extract of
Costus cf. arabicus L leaves (EECAL) over strains of the genus Candida, as well as its inhibitory potential over yeast virulence. Methods: The composition of EECAL was analyzed through High Performance Liquid Chromatography
(HPLC). The Minimum Inhibitory Concentration (MIC) was determined by broth microdilution using spectrophotometer readings and the Minimal Fungicidal Concentration (MFC) was investigated.
The reading data of the MIC was used to trace a cellular growth curve and calculate the Inhibitory Concentration for 50% of the cells (IC50) of the extract and fluconazole. The effect over
the yeast morphology was verified using wet-chamber microculture and visualized through optical microscopy (40x). Results: HPLC detected the presence of flavonoids and phenolic acids. The extract presented fungistatic
effect (MIC of 8.192 µg/mL). The IC50 of the extract and fluconazole varied between 4,008.7 to 5,116.8 µg/mL and 44.0 to 83.1 µg/mL, respectively. The extract inhibited the formation of hyphae
at MICx2 against CA LM 77. For the CA INCQS 40006, the inhibition was verified at MIC/2. In the CT LM 23 and CT INCQS 40042 strains the presence of hyphae was considered absent at the MIC. Conclusion: The extract presented antifungal action on cell growth at elevated concentrations and
an inhibitory effect of dimorphism in the tested Candida species.
Type of Medium:
Online Resource
ISSN:
1570-1808
DOI:
10.2174/1570180815666180827122526
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2019
SSG:
15,3
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