In:
Journal of Medicine and Pharmacy, Hue University of Medicine and Pharmacy
Abstract:
Background: Because of pleasant flavor and aroma as well as cooling-anesthetic effect, menthol and mint oils are widely used in the flavor industry and biocatalytic processes for production of l-menthol are more interested nowadays. Herein, we attempted to develop a biocatalytic process for production of l-menthol through enantioselective hydrolysis of dl-menthyl benzoate using a strain newly isolated from soil.
Methods: From soil samples, we were isolated one strain exhibited the best hydrolytic activity and excellent enantioselectivity as compared to others. So, it was selected as the best enzyme producer and subsequently identified as Acinetobacter sp. based on the 99% similarity of its 16S rDNA sequence, and named Acinetobacter sp. ECU2040. The fermentation process was studied and the fermentation gave about 130 g wet cell, which was equivalent to 18 g dry cell. The catalytic properties of Acinetobacter sp. ECU2040 were also studied. The results showed that the optimum pH and temperature of reaction were 7.5 and 37ºC, respectively. Results and conclusion: The strain Acinetobacter sp. ECU2040 strain exhibited the production of l-menthol ability with the best hydrolytic activity and excellent enantioselectivity.
Key words: l-menthol, enantioselective, isolated strain
Type of Medium:
Online Resource
ISSN:
1859-3836
DOI:
10.34071/jmp.2013.1.9
Language:
Vietnamese
Publisher:
Hue University of Medicine and Pharmacy
Publication Date:
2013
Permalink
