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  • 1
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 103, No. 12 ( 2023-09), p. 5927-5937
    Abstract: Nano starch‐lutein (NS‐L) can be used in three‐dimensional (3D) printed functional surimi. However, the lutein release and printing effect are not ideal. The purpose of this study was to facilitate the function and printing properties of surimi by adding the combination of calcium ion (Ca 2+ ) and NS‐L. RESULTS Printing properties, lutein release and antioxidation of printed Ca 2+ ‐NS‐L‐surimi were determined. The NS‐L‐surimi with 20 mM kg −1 Ca 2+ had the best printing effects (fine accuracy, 99 ± 1%). Compared to NS‐L‐surimi, the structure became denser after adding Ca 2+ , the gel strength, hardness, elasticity, yield stress ( τ ), water holding capacity of Ca 2+ ‐NS‐L‐surimi increased by about 17 ± 4%, 3 ± 1%, 9 ± 2%, 20 ± 4%, 40 ± 5% respectively. These enhanced mechanical strength and self‐supporting ability to resist binding deformation and improve printing accuracy. Moreover, salt dissolution and increased hydrophobic force by Ca 2+ stimulated protein stretching and aggregation, leading to enhancement of gel formation. Decreased printing effects of NS‐L‐surimi with excessive Ca 2+ ( 〉  20 mM kg −1 ) caused by excessive gel strength and τ , leading to strong extrusion force and low extrudability. Additionally, Ca 2+ ‐NS‐L‐surimi had higher digestibility and lutein release rate (increased from 55 ± 2% to 73 ± 3%), because Ca 2+ made NS‐L‐surimi structure porous, which promoted contact of enzyme‐protein. Furthermore, weakened ionic bonds reduced electron binding bondage that combined with released lutein to provide more electrons for enhancing antioxidation. CONCLUSION Collectively, 20 mM kg −1 Ca 2+ could better promote printing process and function exertion of NS‐L‐surimi, facilitating the application of 3D printed functional surimi. © 2023 Society of Chemical Industry.
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2001807-1
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  • 2
    In: Journal of Food Engineering, Elsevier BV, Vol. 346 ( 2023-06), p. 111431-
    Type of Medium: Online Resource
    ISSN: 0260-8774
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2019904-1
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  • 3
    In: Open Forum Infectious Diseases, Oxford University Press (OUP), Vol. 8, No. 10 ( 2021-10-01)
    Abstract: The application of nucleic acid detection methods improves the ability of laboratories to detect diarrheal pathogens, but it also poses new challenges for the interpretation of results. It is often difficult to attribute a diarrhea episode to the detected pathogens. Here we investigated the prevalence of 19 enteropathogens among diarrheal and nondiarrheal children and provided support for understanding the clinical significance of the pathogens. Methods A total of 710 fecal samples were collected from children under 5 years old in 2 different regions of China from May 2017 to March 2018, comprising 383 mild to moderate diarrheal cases and 327 nondiarrheal controls. The enteropathogens were detected using real-time polymerase chain reaction (PCR) or real-time reverse transcription PCR (RT-PCR). Results Enteropathogens were detected in 68.9% of cases and 41.3% of controls. Rotavirus A (adjusted OR [aOR], 9.91; 95% CI, 4.99–19.67), norovirus GI and GII (aOR, 3.82; 95% CI, 2.12–6.89), and Campylobacter jejuni (aOR, 20.12; 95% CI, 2.57–157.38) were significantly associated with diarrhea (P & lt; .05). Adenovirus, norovirus GII, rotavirus A, and enteroaggregative Escherichia coli (pCVD432) gave lower cycle threshold (Ct) values in cases than in controls (P & lt; .05). Rotavirus A and norovirus GII were associated with diarrhea when the Ct values were ≤30 and ≤25, respectively. Conclusions The types and loads of enteropathogens are likely to influence the interpretation of the clinical significance of positive results.
    Type of Medium: Online Resource
    ISSN: 2328-8957
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 2757767-3
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  • 4
    In: Journal of Food Processing and Preservation, Hindawi Limited, Vol. 44, No. 10 ( 2020-10)
    Type of Medium: Online Resource
    ISSN: 0145-8892 , 1745-4549
    URL: Issue
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2175273-4
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  • 5
    Online Resource
    Online Resource
    Elsevier BV ; 2010
    In:  Computers & Mathematics with Applications Vol. 59, No. 2 ( 2010-01), p. 953-963
    In: Computers & Mathematics with Applications, Elsevier BV, Vol. 59, No. 2 ( 2010-01), p. 953-963
    Type of Medium: Online Resource
    ISSN: 0898-1221
    RVK:
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2010
    detail.hit.zdb_id: 2004251-6
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  • 6
    Online Resource
    Online Resource
    American Chemical Society (ACS) ; 2022
    In:  Analytical Chemistry Vol. 94, No. 23 ( 2022-06-14), p. 8174-8180
    In: Analytical Chemistry, American Chemical Society (ACS), Vol. 94, No. 23 ( 2022-06-14), p. 8174-8180
    Type of Medium: Online Resource
    ISSN: 0003-2700 , 1520-6882
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2022
    detail.hit.zdb_id: 1483443-1
    detail.hit.zdb_id: 1508-8
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  • 7
    In: Sustainability, MDPI AG, Vol. 14, No. 6 ( 2022-03-12), p. 3334-
    Abstract: The paradox of Chinese learners refers to a contrast between poor learning approaches and high achievement in China, which reveals a lack of sustainability in Chinese education. In addressing this paradox, Jin Li stood out by studying culture-based learning concepts and providing a comprehensive theoretical framework of the Eastern virtue model versus the Western mind model. However, this framework has not been thoroughly tested in the age of global cultural exchange, and the best learning model for learners has not been determined. This paper used both qualitative (replicating Li’s word association test) and quantitative methods to retest and enrich Li’s theory in present-day China, using four empirical studies. Studies 1 and 2 revealed the influence of global cultural exchange in narrowing the gap between the two models, with appropriate modifications made to Li’s theory. Studies 3 and 4 demonstrated that both of the two models were conducive to students’ academic achievement and creativity, greatly enriching Li’s theory. The implications of achieving a dynamic balance between the virtue model and the mind model to improve the sustainability of Chinese student development are discussed, which contributes to achieving the United Nations’ Sustainable Development Goals.
    Type of Medium: Online Resource
    ISSN: 2071-1050
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518383-7
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  • 8
    Online Resource
    Online Resource
    MDPI AG ; 2023
    In:  Remote Sensing Vol. 15, No. 22 ( 2023-11-16), p. 5384-
    In: Remote Sensing, MDPI AG, Vol. 15, No. 22 ( 2023-11-16), p. 5384-
    Abstract: Data-driven flood susceptibility modeling is an efficient way to map the spatial distribution of flood likelihood. The quality of the flood susceptibility model relies on the learning technique and the data used for learning. The performance of learning techniques has been extensively examined. However, to date, the impact of data sampling strategies has received limited attention. Random sampling is widely favored because of its ease of use. It treats flood-related data as tabular and excludes their spatial dimensions. Flood occurrence is typically uneven over space. Therefore, non-flood sampling should not be completely random. To represent the impact of the spatial dimension, this study proposed a new sampling approach based on spatial dependence, called inverse-occurrence sampling. It selects more non-flood data in low-risk areas than in high-risk areas. The new sampling approach was compared with random and stratified sampling, using six machine learning techniques in two urban areas in Guangzhou, China, with distinct flood mechanisms, that is, Tianhe (flood density 1.5/km2, clustered distribution, average slope 9.02°, downtown district) and Panyu (flood density 0.15/km2, random distribution, average slope 4.55°, suburban district). Learning techniques include support vector machine (SVM), random forest (RF), artificial neural networks (ANNs), convolutional neural networks (CNNs), CNN-SVM, and CNN-RF. The main findings of this study were as follows: (1) Sampling approaches had a greater impact on model performance than learning techniques in terms of area under the receiver operating characteristic curve (AUC). The AUC variations caused by learning techniques ranged from 0.04 to 0.09. Meanwhile, the AUC variations caused by sampling approaches were between 0.15 and 0.22, all larger than 0.1. (2) The new sampling approach outperformed that of the other two sampling approaches for high average AUC values and small AUC variations. The outperformance is robust in regard to multiple learning techniques and different flooding mechanisms. AUCs in the inverse group had a narrower range (0.14–0.18 in Tianhe and 0.35–0.39 in Panyu) than in the random group (0.24–0.28 in Tianhe and 0.43–0.53 in Panyu) and the stratified group (0.23–0.30 in Tianhe and 0.42–0.48 in Panyu). (3) The most accurate learning technique for AUC was CNN-RF, followed by SVM, CNN-SVM, RF, CNN, and ANN. (4) ANN- and CNN-based models tended to produce polarized patterns in flood susceptibility maps, contradicting the ascending order of flood density with increasing susceptibility levels. Flood density outliers tended to appear in the models derived using RF and CNN-RF. Finally, the newly proposed sampling approach is suggested to be applied to flood susceptibility mapping to reflect the impact of spatial dependence.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2513863-7
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  • 9
    In: Chemical Science, Royal Society of Chemistry (RSC), Vol. 14, No. 15 ( 2023), p. 4102-4113
    Abstract: Orthogonal therapy that combines CRISPR-based gene editing and prodrug-based chemotherapy is a promising approach to combat multidrug-resistant cancer. However, its potency to precisely regulate different therapeutic modalities in vivo is limited due to the lack of an integrated platform with high spatiotemporal resolution. Taking advantage of CRISPR technology, a Pt( iv )-based prodrug and orthogonal emissive upconversion nanoparticles (UCNPs), we herein rationally designed the first logic-gated CRISPR-Cas13d-based nanoprodrug for orthogonal photomodulation of gene editing and prodrug release for enhanced cancer therapy. The nanoprodrug (URL) was constructed by encapsulating a green light-activatable Pt( iv ) prodrug and UV light-activatable Cas13d gene editing tool into UCNPs. We demonstrated that URL maintained excellent orthogonal emission behaviors under 808 and 980 nm excitations, allowing wavelength-selective photoactivation of Cas13d and the prodrug for downregulation of the resistance-related gene and induction of chemo-photodynamic therapy, respectively. Moreover, the photomodulation superiority of URL for overcoming drug resistance was highlighted by integrating it with a Boolean logic gate for programmable modulation of multiple cell behaviors. Importantly, in vivo studies demonstrated that URL can promote Pt( iv ) prodrug activation and ROS generation and massively induce on-target drug accumulation by Cas13d-mediated drug resistance attenuation, delivering an ultimate chemo-photodynamic therapeutic performance in efficiently eradicating primary tumors and preventing further liver metastasis. Collectively, our results suggest that URL expands the Cas13d-based genome editing toolbox into prodrug nanomedicine and accelerates the discovery of new orthogonal therapeutic approaches.
    Type of Medium: Online Resource
    ISSN: 2041-6520 , 2041-6539
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2023
    detail.hit.zdb_id: 2559110-1
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  • 10
    In: Journal of Food Process Engineering, Wiley, Vol. 46, No. 3 ( 2023-03)
    Abstract: The gel is an important factor in 3D printability of Pennahia argentata surimi. While surimi gel is sensitive to temperature and influenced by chemical bonds changes. Hence, preheating effects on printing and material properties of surimi were investigated. Results shown hydrogen bonds and ionic bonds contents in surimi under different heating conditions decreased about 20%–90%. Meanwhile, hydrophobic force and disulfide bonds increased about 0.2–6 times. These changes formed more gel, as well as 6.5% at most immovable water transferred into free water which enhanced texture properties and made solid properties of rheology in surimi dominant. This phenomenon occurred significantly in temperature range of gel formation (35–40°C, 90°C) while became extinct at 45°C with 10–30 min due to gel degradation. These made surimi by heating exhibit strong mechanical strength, which is conducive to forming process when printing after extrusion. However, if they occurred before extrusion, these could increase extrusion difficulty to make filamentous materials be broken easily after extrusion, weakening printing effect. Hence, preheating of surimi before extrusion was not suitable for 3D printing. While short‐time heating during or after extrusion might be more appropriate for printed surimi, promoting the application of surimi in 3D printing technology. Practical Applications 3D printing is a new technology in food processing that refers the process of connecting or curing materials to form 3D objects by continuously adding layers of materials. Surimi is one of the suitable materials which can promote the process of printing. While the material properties of surimi are affected by temperature that influences the printing effects. The purpose of this study was to investigate the effects of different temperatures on the material properties of Pennahia argentata surimi, and determine the suitable heating process of printed surimi products. These results could promote the application of 3D printing technology in surimi processing.
    Type of Medium: Online Resource
    ISSN: 0145-8876 , 1745-4530
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2175259-X
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