In:
Journal of the American Oil Chemists' Society, Wiley, Vol. 69, No. 4 ( 1992-04), p. 355-358
Abstract:
Homogenates of microspore‐derived embryos of rape ( Brassica napus L.) incubated with [1‐ 14 C]erucoyl‐CoA and 1,2‐dierucoylglycerol are able to assemble trierucoyl‐glycerol (trierucin). In addition, radioactive triacylglycerols are formed by transferring [1‐ 14 C]‐erucoyl moieties to endogenous lipid precursors. Stereospecific analysis of radioactive triacylglycerols revealed that labeled erucoyl moieties had been incorporated exclusively into the sn ‐1,3 positions with more than 95% of radioactivity in the sn ‐3 position. No incorporation of labeled erucic acid into the sn ‐2 position has been observed. All data agree with the involvement of 1,2‐diacylglycerol acyltransferase (E.C. 2.3.1.20), which utilized 1,2‐dierucoylglycerol as well as endogenous 1,2‐diacylglycerols as acceptors of erucoyl moieties. This result is of particular interest for the genetic modification of rape and other Cruciferae for producing trierucin in their seed oils.
Type of Medium:
Online Resource
ISSN:
0003-021X
,
1558-9331
Language:
English
Publisher:
Wiley
Publication Date:
1992
detail.hit.zdb_id:
2041388-9
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