In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 5 ( 2023-5-19), p. e3002124-
Abstract:
Necrotizing enterocolitis (NEC) is a gastrointestinal complication of premature infants with high rates of morbidity and mortality. A comprehensive view of the cellular changes and aberrant interactions that underlie NEC is lacking. This study aimed at filling in this gap. We combine single-cell RNA sequencing (scRNAseq), T-cell receptor beta (TCRβ) analysis, bulk transcriptomics, and imaging to characterize cell identities, interactions, and zonal changes in NEC. We find an abundance of proinflammatory macrophages, fibroblasts, endothelial cells as well as T cells that exhibit increased TCRβ clonal expansion. Villus tip epithelial cells are reduced in NEC and the remaining epithelial cells up-regulate proinflammatory genes. We establish a detailed map of aberrant epithelial–mesenchymal–immune interactions that are associated with inflammation in NEC mucosa. Our analyses highlight the cellular dysregulations of NEC-associated intestinal tissue and identify potential targets for biomarker discovery and therapeutics.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002124
DOI:
10.1371/journal.pbio.3002124.g001
DOI:
10.1371/journal.pbio.3002124.g002
DOI:
10.1371/journal.pbio.3002124.g003
DOI:
10.1371/journal.pbio.3002124.g004
DOI:
10.1371/journal.pbio.3002124.g005
DOI:
10.1371/journal.pbio.3002124.g006
DOI:
10.1371/journal.pbio.3002124.s001
DOI:
10.1371/journal.pbio.3002124.s002
DOI:
10.1371/journal.pbio.3002124.s003
DOI:
10.1371/journal.pbio.3002124.s004
DOI:
10.1371/journal.pbio.3002124.s005
DOI:
10.1371/journal.pbio.3002124.s006
DOI:
10.1371/journal.pbio.3002124.s007
DOI:
10.1371/journal.pbio.3002124.s008
DOI:
10.1371/journal.pbio.3002124.s009
DOI:
10.1371/journal.pbio.3002124.s010
DOI:
10.1371/journal.pbio.3002124.s011
DOI:
10.1371/journal.pbio.3002124.s012
DOI:
10.1371/journal.pbio.3002124.s013
DOI:
10.1371/journal.pbio.3002124.s014
DOI:
10.1371/journal.pbio.3002124.s015
DOI:
10.1371/journal.pbio.3002124.s016
DOI:
10.1371/journal.pbio.3002124.s017
DOI:
10.1371/journal.pbio.3002124.r001
DOI:
10.1371/journal.pbio.3002124.r002
DOI:
10.1371/journal.pbio.3002124.r003
DOI:
10.1371/journal.pbio.3002124.r004
DOI:
10.1371/journal.pbio.3002124.r005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X
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