In:
Journal of Biological Rhythms, SAGE Publications, Vol. 11, No. 4 ( 1996-12), p. 311-316
Abstract:
The purpose of the present study was to determine whether there is a rhythm in glutamic acid decarboxylase (GAD) message in the suprachiasmatic nucleus (SCN) of rats housed in a light:dark cycle. The mRNAs encoding two isoforms of GAD (i.e., GAD 65 and GAD 67 ) were examined using in situ hybridization histochemistry. Computerized image analysis of film autoradiographs revealed that GAD 65 mRNA was significantly higher in the light than it was in the dark. GAD 67 mRNA levels were lower overall and did not decrease significantly in the dark. Following emulsion autoradiography, silver grain counts over individual SCN cells indicated that GAD 65 mRNA was highest in the dorsomedial hypothalamus during the light. These data suggest that GAD mRNA varies rhythmically in the SCN and that mRNA levels are regulated differently within SCN subdivisions during the light:dark cycle.
Type of Medium:
Online Resource
ISSN:
0748-7304
,
1552-4531
DOI:
10.1177/074873049601100404
Language:
English
Publisher:
SAGE Publications
Publication Date:
1996
detail.hit.zdb_id:
2018064-0
SSG:
12
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