In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 5 ( 2022-5-5), p. e0264453-
Abstract:
Soybean is a legume crop enriched with proteins and oil. It is frequently exposed to anthropogenic and natural flooding that limits its growth and yield. Current study applied gel-free proteomic techniques to unravel soybean response mechanism to flooding stress. Two-days-old soybeans were flooded for 4 days continuously and root samples were collected at days 2 to 6 for proteomic and enzymatic analyses. Age-matched untreated soybeans were collected as control. After protein extraction, purification and tryptic digestion, the peptides were analyzed on nano-liquid chromatography-mass spectrometry. A total of 539 and 472 proteins with matched peptides 2 or more were identified in control and flooded seedlings, respectively. Among these 364 proteins were commonly identified in both control and flooded soybeans. Fourty-two protein’s abundances were changed 4-fold after 2-days of flooding stress as compared to starting point. The cluster analysis showed that highly increased proteins included cupin family proteins, enolase, pectin methylesterase inhibitor, glyoxalase II, alcohol dehydrogenase and aldolase. The enzyme assay of enolase and pectin methylesterase inhibitor confirmed protein abundance changes. These findings suggest that soybean adopts the less energy consuming strategies and brings biochemical and structural changes in the cell wall to effectively respond to flooding stress and for the survival.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0264453
DOI:
10.1371/journal.pone.0264453.g001
DOI:
10.1371/journal.pone.0264453.g002
DOI:
10.1371/journal.pone.0264453.g003
DOI:
10.1371/journal.pone.0264453.g004
DOI:
10.1371/journal.pone.0264453.g005
DOI:
10.1371/journal.pone.0264453.g006
DOI:
10.1371/journal.pone.0264453.g007
DOI:
10.1371/journal.pone.0264453.t001
DOI:
10.1371/journal.pone.0264453.s001
DOI:
10.1371/journal.pone.0264453.s002
DOI:
10.1371/journal.pone.0264453.s003
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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