In:
Enzyme Research, Hindawi Limited, Vol. 2011 ( 2011-09-15), p. 1-11
Abstract:
The production of flavonoid glycosides by removing rhamnose from
rutinosides can be accomplished through enzymatic catalysis. Naringinase is an enzyme complex, expressing both α -L-rhamnosidase and β -D-glucosidase activities, with application in glycosides
hydrolysis. To produce monoglycosylated flavonoids with naringinase, the expression of β -D-glucosidase activity is not desirable leading to the
need of expensive methods for α -L-rhamnosidase purification. Therefore, the main purpose
of this study was the inactivation of β -D-glucosidase activity expressed by naringinase keeping α -L-rhamnosidase with a high retention activity. Response
surface methodology (RSM) was used to evaluate the effects of temperature and pH on β -D-glucosidase inactivation. A selective inactivation of β -D-glucosidase activity of naringinase was achieved at 81.5 ∘ C and pH 3.9, keeping a very high residual activity of α -L-rhamnosidase (78%). This was a crucial achievement
towards an easy and cheap production method of very expensive flavonoids, like prunin and isoquercetin starting from naringin and
rutin, respectively.
Type of Medium:
Online Resource
ISSN:
2090-0414
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2011
detail.hit.zdb_id:
2573712-0
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