In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. Pt_7 ( 2012-07-01), p. 1666-1673
Abstract:
The first alkaliphilic obligately anaerobic hydrogenotrophic homoacetogenic bacterium, strain Z-7100 T , was isolated from sediments of the soda-depositing soda lake Tanatar III (Altay, Russia). Cells were thin, flexible rods, motile, Gram-negative and spore-forming. The organism was an obligate alkaliphile, growing at pH 8.5 to 10.5, with optimum growth at pH 8.8–9.3, and it grew in soda brines containing 1.9–4.7 M total Na + (optimum at 2.8–3.3 M). It exhibited an obligate dependence upon sodium carbonate but not upon chloride ions with an NaCl range for growth of 0–14 % (w/v) and an optimum at 7.0–8.5 % (w/v). The isolate was mesophilic and grew at temperatures from 25 to 45 °C, with an optimum at 40 °C. An H 2 +CO 2 mixture, ethanol, pyruvate and lactate were utilized with the formation of acetate as the sole metabolic product. Carbohydrates and amino acids did not support growth. The isolate had a respiratory type of metabolism, reducing NO 3 – , SeO 4 2− or anthraquinone-2,6-disulfonate (as electron acceptors with ethanol as an electron donor). It was able to grow chemolithotrophically on H 2 +CO 2 in medium supplemented with a vitamin solution only. The major cellular fatty acids were the saturated fatty acids anteiso-C 15 , C 14 : 0 and C 16 : 0 and the aldehydes C 16 , C 14 and anteiso-C 15 . The DNA G+C content of the isolate was 32.0 mol%. 16S rRNA gene sequence analysis showed that strain Z-7100 T is a member of the order Halanaerobiales and represents a new branch within the family Halobacteroidaceae , clustering with the type strain of Selenihalanaerobacter shriftii (92.9 % gene sequence similarity). On the basis of its physiological characteristics and phylogenetic position, the isolate is considered to represent a novel species in a new genus within the family Halobacteroidaceae . The name Fuchsiella alkaliacetigena gen. nov., sp. nov. is proposed. The type strain of the type species is Z-7100 T ( = DSM 24880 T = VKM B-2667 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.034363-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2012
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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