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  • 1
    Online Resource
    Online Resource
    Influence of Different Light-Emitting Diode Colors on Growth and Phycobiliprotein Generation of Arthrospira platensis
    MDPI AG ; 2022
    In:  Life Vol. 12, No. 6 ( 2022-06-15), p. 895-
    Details
    In: Life, MDPI AG, Vol. 12, No. 6 ( 2022-06-15), p. 895-
    Abstract: Light-emitting diodes (LED) can be utilized as tailorable artificial light sources for the cultivation of cyanobacteria such as Arthrospira platensis (AP). To study the influence of different LED light colors on phototrophic growth and biomass composition, AP was cultured in closed bioreactors and exposed to red, green, blue, or white LED lights. The illumination with red LED light resulted in the highest cell growth and highest cell densities compared to all other light sources (order of cell densities: red 〉 white 〉 green 〉 blue LED light). In contrast, the highest phycocyanin concentrations were found when AP was cultured under blue LED light (e.g., order of concentrations: blue 〉 white 〉 red 〉 green LED light). LED-blue light stimulated the accumulation of nitrogen compounds in the form of phycobiliproteins at the expense of cell growth. The results of the study revealed that exposure to different LED light colors can improve the quality and quantity of the biomass gained in AP cultures.
    Type of Medium: Online Resource
    ISSN: 2075-1729
    URL: Article
    DOI: 10.3390/life12060895
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662250-6
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  • 2
    Online Resource
    Online Resource
    Morphology and Growth of Arthrospira platensis during Cultivation in a Flat-Type Bioreactor
    MDPI AG ; 2021
    In:  Life Vol. 11, No. 6 ( 2021-06-09), p. 536-
    Details
    In: Life, MDPI AG, Vol. 11, No. 6 ( 2021-06-09), p. 536-
    Abstract: Arthrospira platensis (AP) is a cyanobacterium with a high economic value and is nowadays one of the most important industrially cultivated microalgae. Knowledge of its growth is essential for the understanding of its physiology and yield. The growth of AP biomass occurs through two mechanisms: (1) propagation by fragmentation of trichomes, and (2) the trichomes are extended by binary fission until they reach their mature status. These phases are visualized by live cell light and laser scanning microscopy, demonstrating the different phases of AP growth.
    Type of Medium: Online Resource
    ISSN: 2075-1729
    URL: Article
    DOI: 10.3390/life11060536
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2662250-6
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  • 3
    Online Resource
    Online Resource
    Lipophilic and Hydrophilic Compounds from Arthrospira platensis and Its Effects on Tissue and Blood Cells—An Overview
    MDPI AG ; 2022
    In:  Life Vol. 12, No. 10 ( 2022-09-26), p. 1497-
    Details
    In: Life, MDPI AG, Vol. 12, No. 10 ( 2022-09-26), p. 1497-
    Abstract: The cyanobacterium Arthrospira platensis (Spirulina platensis) is a natural source of considerable amounts of ingredients that are relevant for nutra- and pharmaceutical uses. Different hydrophilic and hydrophobic substances can be obtained by extraction from the biomass. The respective extraction techniques determine the composition of substances in the extract and thus its biological activity. In this short review, we provide an overview of the hydrophilic compounds (phenols, phycobiliproteins, polysaccharides, and vitamins) and lipophilic ingredients (chlorophylls, vitamins, fatty acids, and glycolipids) of Arthrospira platensis. The principal influences of these substances on blood and tissue cells are briefly summarized.
    Type of Medium: Online Resource
    ISSN: 2075-1729
    URL: Article
    DOI: 10.3390/life12101497
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662250-6
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  • 4
    Online Resource
    Online Resource
    Cold Atmospheric Pressure Plasma in Wound Healing and Cancer Treatment
    MDPI AG ; 2020
    In:  Applied Sciences Vol. 10, No. 19 ( 2020-10-01), p. 6898-
    Details
    In: Applied Sciences, MDPI AG, Vol. 10, No. 19 ( 2020-10-01), p. 6898-
    Abstract: Plasma medicine is gaining increasing attention and is moving from basic research into clinical practice. While areas of application are diverse, much research has been conducted assessing the use of cold atmospheric pressure plasma (CAP) in wound healing and cancer treatment—two applications with entirely different goals. In wound healing, a tissue-stimulating effect is intended, whereas cancer therapy aims at killing malignant cells. In this review, we provide an overview of the latest clinical and some preclinical research on the efficacy of CAP in wound healing and cancer therapy. Furthermore, we discuss the current understanding of molecular signaling mechanisms triggered by CAP that grant CAP its antiseptic and tissue regenerating or anti-proliferative and cell death-inducing properties. For the efficacy of CAP in wound healing, already substantial evidence from clinical studies is available, while evidence for therapeutic effects of CAP in oncology is mainly from in vitro and in vivo animal studies. Efforts to elucidate the mode of action of CAP suggest that different components, such as ultraviolet (UV) radiation, electromagnetic fields, and reactive species, may act synergistically, with reactive species being regarded as the major effector by modulating complex and concentration-dependent redox signaling pathways.
    Type of Medium: Online Resource
    ISSN: 2076-3417
    URL: Article
    DOI: 10.3390/app10196898
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2704225-X
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  • 5
    Online Resource
    Online Resource
    Effect of Prostanoids on Human Platelet Function: An Overview
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 21, No. 23 ( 2020-11-27), p. 9020-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 21, No. 23 ( 2020-11-27), p. 9020-
    Abstract: Prostanoids are bioactive lipid mediators and take part in many physiological and pathophysiological processes in practically every organ, tissue and cell, including the vascular, renal, gastrointestinal and reproductive systems. In this review, we focus on their influence on platelets, which are key elements in thrombosis and hemostasis. The function of platelets is influenced by mediators in the blood and the vascular wall. Activated platelets aggregate and release bioactive substances, thereby activating further neighbored platelets, which finally can lead to the formation of thrombi. Prostanoids regulate the function of blood platelets by both activating or inhibiting and so are involved in hemostasis. Each prostanoid has a unique activity profile and, thus, a specific profile of action. This article reviews the effects of the following prostanoids: prostaglandin-D2 (PGD2), prostaglandin-E1, -E2 and E3 (PGE1, PGE2, PGE3), prostaglandin F2α (PGF2α), prostacyclin (PGI2) and thromboxane-A2 (TXA2) on platelet activation and aggregation via their respective receptors.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms21239020
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 23 ( 2022-11-29), p. 14987-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 23 ( 2022-11-29), p. 14987-
    Abstract: Guided bone regeneration (GBR) has become a clinically standard modality for the treatment of localized jawbone defects. Barrier membranes play an important role in this process by preventing soft tissue invasion outgoing from the mucosa and creating an underlying space to support bone growth. Different membrane types provide different biological mechanisms due to their different origins, preparation methods and structures. Among them, collagen membranes have attracted great interest due to their excellent biological properties and desired bone regeneration results to non-absorbable membranes even without a second surgery for removal. This work provides a comparative summary of common barrier membranes used in GBR, focusing on recent advances in collagen membranes and their biological mechanisms. In conclusion, the review article highlights the biological and regenerative properties of currently available barrier membranes with a particular focus on bioresorbable collagen-based materials. In addition, the advantages and disadvantages of these biomaterials are highlighted, and possible improvements for future material developments are summarized.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms232314987
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Design of a Medium Voltage Generator with DC-Cascade for High Power Wind Energy Conversion Systems
    MDPI AG ; 2021
    In:  Energies Vol. 14, No. 11 ( 2021-05-26), p. 3106-
    Details
    In: Energies, MDPI AG, Vol. 14, No. 11 ( 2021-05-26), p. 3106-
    Abstract: This paper shows a new concept to generate medium voltage (MV) in wind power application to avoid an additional transformer. Therefore, the generator must be redesigned with additional constraints and a new topology for the power rectifier system by using multiple low voltage (LV) power rectifiers connected in series and parallel to increase the DC output voltage. The combination of parallel and series connection of rectifiers is further introduced as DC-cascade. With the resulting DC-cascade, medium output voltage is achieved with low voltage rectifiers and without a bulky transformer. This approach to form a DC-cascade reduces the effort required to achieve medium DC voltage with a simple rectifier system. In this context, a suitable DC-cascade control was presented and verified with a laboratory test setup. A gearless synchronous generator, which is highly segmented so that each segment can be connected to its own power rectifier, is investigated. Due to the mixed AC and DC voltage given by the DC-cascade structure, it becomes more demanding to the design of the generator insulation, which influences the copper fill factor and the design of the cooling system. A design strategy for the overall generator design is carried out considering the new boundary conditions.
    Type of Medium: Online Resource
    ISSN: 1996-1073
    URL: Article
    DOI: 10.3390/en14113106
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2437446-5
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  • 8
    Online Resource
    Online Resource
    Metabolic Response to XD14 Treatment in Human Breast Cancer Cell Line MCF-7
    MDPI AG ; 2016
    In:  International Journal of Molecular Sciences Vol. 17, No. 10 ( 2016-10-24), p. 1772-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 17, No. 10 ( 2016-10-24), p. 1772-
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms17101772
    Language: English
    Publisher: MDPI AG
    Publication Date: 2016
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Combining Biocompatible and Biodegradable Scaffolds and Cold Atmospheric Plasma for Chronic Wound Regeneration
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 17 ( 2021-08-25), p. 9199-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 17 ( 2021-08-25), p. 9199-
    Abstract: Skin regeneration is a quite complex process. Epidermal differentiation alone takes about 30 days and is highly regulated. Wounds, especially chronic wounds, affect 2% to 3% of the elderly population and comprise a heterogeneous group of diseases. The prevailing reasons to develop skin wounds include venous and/or arterial circulatory disorders, diabetes, or constant pressure to the skin (decubitus). The hallmarks of modern wound treatment include debridement of dead tissue, disinfection, wound dressings that keep the wound moist but still allow air exchange, and compression bandages. Despite all these efforts there is still a huge treatment resistance and wounds will not heal. This calls for new and more efficient treatment options in combination with novel biocompatible skin scaffolds. Cold atmospheric pressure plasma (CAP) is such an innovative addition to the treatment armamentarium. In one CAP application, antimicrobial effects, wound acidification, enhanced microcirculations and cell stimulation can be achieved. It is evident that CAP treatment, in combination with novel bioengineered, biocompatible and biodegradable electrospun scaffolds, has the potential of fostering wound healing by promoting remodeling and epithelialization along such temporarily applied skin replacement scaffolds.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms22179199
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    X-ray Computed Tomography Method for Macroscopic Structural Property Evaluation of Active Twist Composite Blades
    MDPI AG ; 2021
    In:  Aerospace Vol. 8, No. 12 ( 2021-11-30), p. 370-
    Details
    In: Aerospace, MDPI AG, Vol. 8, No. 12 ( 2021-11-30), p. 370-
    Abstract: This paper describes an evaluation of the structural properties of the next-generation active twist blade using X-ray computed tomography (CT) combined with digital image processing. This non-destructive testing technique avoids the costly demolition of the blade structure. The CT scan covers the whole blade region, including the root, transition, and tip regions, as well as the airfoil blade regions, in which there are spanwise variations in the interior structural layout due to the existence of heavy instrumentation. The three-dimensional digital image data are processed at selected radial stations, and finite element beam cross-section analyses are conducted to evaluate the structural properties of the blade at the macroscopic level. The fidelity of the digital blade model is first assessed by correlating the estimated blade mass with the measured data. A separate mechanical measurement is then carried out to determine the representative elastic properties of the blade and to verify the predicted results. The agreement is found to be good to excellent for the mass, elastic axis, flap bending, and torsional rigidity. The discrepancies are less than 2.0% for the mass and elastic axis locations, and about 8.1% for the blade stiffness properties, as compared with the measured data. Finally, a sensitivity analysis is conducted to clarify the impact of modeling the sensor and actuator cables, nose weight, and manufacturing imperfections on the structural properties of the blade.
    Type of Medium: Online Resource
    ISSN: 2226-4310
    URL: Article
    DOI: 10.3390/aerospace8120370
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2756091-0
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