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  • Canadian Science Publishing  (4)
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  • Canadian Science Publishing  (4)
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  • English  (4)
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  • 1
    Online Resource
    Online Resource
    Lipopolysaccharide-pretreated mesenchymal stem cell-conditioned medium optimized with 10 kDa filter attenuates the injury of H9c2 cardiomyocytes in a model of hypoxia/reoxygenation
    Canadian Science Publishing ; 2022
    In:  Canadian Journal of Physiology and Pharmacology Vol. 100, No. 7 ( 2022-07-01), p. 651-664
    Details
    In: Canadian Journal of Physiology and Pharmacology, Canadian Science Publishing, Vol. 100, No. 7 ( 2022-07-01), p. 651-664
    Abstract: Mesenchymal stem cell-derived conditioned medium (MSC-CM) improves cardiac function, which is partly attributed to the released paracrine factors. Since such cardioprotection is moderate and transient, it is essential that MSC-CM's effective components are optimized to alleviate myocardial injury. To optimize MSC-CM, MSCs were treated with or without lipopolysaccharides (LPSs) for 48 h (serum-free), and the supernatant was collected. Then, LPS-CM (MSC stimulated by LPS) was further treated with LPS remover (LPS Re-CM) or was concentrated with a 10 kDa cutoff filter (10 kDa-CM). Enzyme-linked immunosorbent assay showed that all the pretreatments increased the levels of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin growth factor (IGF) except LPS Re-CM; 10 kDa-CM was superior to the other CMs. Cell Counting Kit-8 displayed that the viability of injured H9c2 cells was enhanced with the increase in the MSC-CM concentration. We also found that the 10 kDa-CM significantly alleviated H9c2 hypoxia/reoxygenation (H/R) injury, as evidenced by the increased Bcl-2/Bax ratio, and decreased the levels of lactate dehydrogenase and cardiac troponin. Transmission electron microscopy (TEM), TdT-mediated dUTP nick-end labelling (TUNEL), and hematoxylin and eosin staining (H & E) confirmed that 10 kDa-CM inhibited H/R-induced H9c2 morphological changes. Proteomic analysis identified 41 differentially expressed proteins in 10 kDa-CM, among which anti-inflammation, proangiogenesis, and antiapoptosis were related to cardiac protection. This study indicates that 10 kDa-CM protects H9c2 cardiomyocytes from H/R injury by preserving most of the protective factors, such as VEGF, HGF, and IGF, in MSC-CM.
    Type of Medium: Online Resource
    ISSN: 0008-4212 , 1205-7541
    URL: Article
    DOI: 10.1139/cjpp-2021-0745
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2022
    detail.hit.zdb_id: 2004356-9
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  • 2
    Online Resource
    Online Resource
    Correction: Lipopolysaccharide-pretreated mesenchymal stem cell-conditioned medium optimized with 10 kDa filter attenuates the injury of H9c2 cardiomyocytes in a model of hypoxia/reoxygenation
    Canadian Science Publishing ; 2022
    In:  Canadian Journal of Physiology and Pharmacology Vol. 100, No. 11 ( 2022-11-01), p. 1086-1086
    Details
    In: Canadian Journal of Physiology and Pharmacology, Canadian Science Publishing, Vol. 100, No. 11 ( 2022-11-01), p. 1086-1086
    Type of Medium: Online Resource
    ISSN: 0008-4212 , 1205-7541
    URL: Article
    DOI: 10.1139/cjpp-2022-0418
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2022
    detail.hit.zdb_id: 2004356-9
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  • 3
    Online Resource
    Online Resource
    Attenuation of hepatic steatosis by purple sweet potato colour is associated with blocking Src/ERK/C/EBPβ signalling in high-fat-diet–treated mice
    Canadian Science Publishing ; 2017
    In:  Applied Physiology, Nutrition, and Metabolism Vol. 42, No. 10 ( 2017-10), p. 1082-1091
    Details
    In: Applied Physiology, Nutrition, and Metabolism, Canadian Science Publishing, Vol. 42, No. 10 ( 2017-10), p. 1082-1091
    Abstract: Our previous work showed that purple sweet potato colour (PSPC), a class of naturally occurring anthocyanins, effectively improved hepatic glucose metabolic dysfunction in high-fat-diet (HFD)–treated mice. This study investigated the effects of PSPC on HFD-induced hepatic steatosis and the signalling events associated with these effects. Mice were divided into 4 groups: control group, HFD group, HFD+PSPC group, and PSPC group. PSPC was administered daily for 20 weeks at oral doses of 700 mg/(kg·day) −1 ). Our results showed that PSPC significantly improved obesity and related metabolic parameters, as well as liver injury in HFD-treated mice. Moreover, PSPC dramatically attenuated hepatic steatosis in HFD-treated mice. PSPC markedly prevented oxidative stress-mediated Src activation in HFD-treated mouse livers. Furthermore, PSPC feeding remarkably suppressed mitogen-activated protein kinase kinase/extracellular-signal-regulated kinase (MEK/ERK) signalling and consequent CCAAT/enhancer binding protein β (C/EBPβ) activation and restored AMPK activation in HFD-treated mouse livers, which was confirmed by U0126 treatment. Ultimately, PSPC feeding dramatically reduced protein expression of FAS and CD36 and the activation of ACC, and increased the protein expression of CPT1A in the livers of HFD-treated mice, indicating decreased lipogenesis and fatty acid uptake and enhanced fatty acid oxidation. In conclusion, PSPC exhibited beneficial effects on hepatic steatosis, which were associated with blocking Src and C/EBPβ activation.
    Type of Medium: Online Resource
    ISSN: 1715-5312 , 1715-5320
    URL: Article
    DOI: 10.1139/apnm-2016-0635
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2017
    SSG: 31
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  • 4
    Online Resource
    Online Resource
    Effects of regulated deficit irrigation on grain yield and water use efficiency of spring wheat in an arid environment
    Canadian Science Publishing ; 2005
    In:  Canadian Journal of Plant Science Vol. 85, No. 4 ( 2005-10-01), p. 829-837
    Details
    In: Canadian Journal of Plant Science, Canadian Science Publishing, Vol. 85, No. 4 ( 2005-10-01), p. 829-837
    Abstract: Grain yield and water use efficiency (WUE) of spring wheat ( Triticum aestivum L.) in arid environments can be improved by applying irrigation selectively to allow soil water deficits to develop at non-critical stages of crop development. Field experiments were conducted on a loam soil in Zhangye district, northwest China in 2003 and 2004 to determine the grain yield, yield components, and water use characteristics of spring wheat in response to regulated deficit irrigation (RDI) schemes. Wheat grown under the RDI schemes produced 17% (in 2004) and 29% (in 2003) higher grain yield than wheat grown under water deficit-free control (5.6 t ha -1 in 2003 and 6.2 t ha -1 in 2004). Among six RDI schemes studied, wheat having a high water deficit at the jointing stage, but free from water def icit from booting to grain-filling produced highest grain yield in both 2003 (7.95 t ha -1 ) and 2004 (7.26 t ha -1 ). Compared with the control, wheat plants grown under the RDI schemes received 59 mm (or 15%) less water via irrigation, but they either extrac ted 41 mm more (or 74%) water from the soil profile (in 2003) or lowered (16%) evapotranspiration (ET) (in 2004). Grain yield increased as ET increased from 415 to 460 mm, and declined beyond 460 mm. The WUE values varied from 0.0116 to 0.0168 t ha -1 mm -1 , and wheat grown under the RDI had 26% greater WUE compared with the control. Grain yield and WUE of spring wheat can be greatly improved by regulated deficit irrigation with reduced amounts of water. This practice is particularly valuable in arid regions where wheat production relies heavily on irrigation. Key words:
    Type of Medium: Online Resource
    ISSN: 0008-4220 , 1918-1833
    URL: Article
    DOI: 10.4141/P04-165
    Language: English
    Publisher: Canadian Science Publishing
    Publication Date: 2005
    detail.hit.zdb_id: 2016989-9
    SSG: 12
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