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  • Canadian Science Publishing  (5)
  • Englisch  (5)
  • Biologie  (5)
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  • Canadian Science Publishing  (5)
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  • Englisch  (5)
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  • Biologie  (5)
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  • 1
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2017
    In:  Canadian Journal of Microbiology Vol. 63, No. 1 ( 2017-01), p. 1-10
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 63, No. 1 ( 2017-01), p. 1-10
    Kurzfassung: Secoisolariciresinol (SECO) is a lignan of potential therapeutic value for diseases such as cancer, but its use has been limited by the lack of ideal production methods, even though its precursors are abundant in plants, such as flaxseeds. Here, we report the characterization of a bacterial strain, S1, isolated from the human intestinal flora, which could produce secoisolariciresinol by biotransformation of precursors in defatted flaxseeds. This bacterium was a Gram-negative and facultatively anaerobic straight rod without capsules. Biochemical assays showed that it was negative for production of oxidase, lysine decarboxylase, ornithine decarboxylase, arginine dihydrolase, and β-glucolase. The G + C content of genomic DNA was 57.37 mol%. Phylogenetic analysis by 16S rRNA and rpoB gene sequences demonstrated S1’s close relatedness to Klebsiella. No homologues were found for wzb or wzc (capsular genes), which may explain why Klebsiella sp. strain S1 does not have the capsule and was isolated from a healthy human individual. Based on the percentages of homologous genes with identical nucleotide sequences between the bacteria in comparison, we found that clear-cut genetic boundaries had been formed between S1 and any other Klebsiella strains compared, dividing them into distinct phylogenetic lineages. This work demonstrates that the intestinal Klebsiella, well known as important opportunistic pathogens prevalent in potentially fatal nosocomial infections, may contain lineages that are particularly beneficial to the human health.
    Materialart: Online-Ressource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2017
    ZDB Id: 280534-0
    ZDB Id: 1481972-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2020
    In:  Canadian Journal of Microbiology Vol. 66, No. 1 ( 2020-01), p. 59-70
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 66, No. 1 ( 2020-01), p. 59-70
    Kurzfassung: In this study, we explored the effect of chemical oxygen demand (COD) load on the nitrification and microbial communities in activated sludge isolated from an aerobic nitrifying tank. The activated sludge was cultured in three different COD groups: L-COD, 200 mg/L; M-COD, 1200 mg/L; H-COD, 4200 mg/L. The results indicated that the COD exerts a negligible effect on the nitrogen removal ability within the first 24 h. However, the nitrification rate decreased with culture time; the ammonium degradation rates were found to be 80.26%, 57.56%, and 43.43% at 72 h in the three COD groups, respectively. These values correspond to decreases of 19.40%, 41.83%, and 51.48%, respectively, in relation to those observed at 24 h. The activated sludge in the different COD groups exhibited similar community compositions after 24 h, as assessed by Illumina high-throughput sequencing, while a significant difference in the relative abundances of some organisms occurred after 48 and 72 h. Proteobacteria was the main phylum, with a relative abundance of 〉 51.45%. The genera Aridibacter, Paracoccus, Nitrospira, and Nitrosomonas were suppressed by COD load over time. This study may contribute to our knowledge about the nitrification ability and microbial communities in activated sludge at different COD load levels.
    Materialart: Online-Ressource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2020
    ZDB Id: 280534-0
    ZDB Id: 1481972-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2015
    In:  Canadian Journal of Microbiology Vol. 61, No. 9 ( 2015-09), p. 691-699
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 61, No. 9 ( 2015-09), p. 691-699
    Kurzfassung: Benzene, toluene, ethylbenzene, and xylene (BTEX) are of great environmental concern because of their widespread occurrence in groundwater and soil, posing an increasing threat to human health. The aerobic denitrifying BTEX-degrading bacterium Pseudomonas thivervalensis MAH1 was isolated from BTEX-contaminated sediment under nitrate-reducing conditions. The degradation rates of benzene, toluene, ethylbenzene, and xylene by strain MAH1 were 4.71, 6.59, 5.64, and 2.59 mg·L −1 ·day −1 , respectively. The effects of sodium citrate, nitrate, and NaH 2 PO 4 on improving BTEX biodegradation were investigated, and their optimum concentrations were 0.5 g·L −1 , 100 mg·L −1 , and 0.8 mmol·L −1 , respectively. Moreover, MAH1, which has nirS and nosZ genes, removed ammonium, nitrate, and nitrite at 2.49 mg NH 4 + -N·L −1 ·h −1 , 1.50 mg NO 3 − -N·L −1 ·h −1 , and 0.83 mg NO 2 − -N·L −1 ·h −1 , respectively. MAH1 could help in mitigating the pollution caused by nitrogen amendments for biostimulation. This study highlighted the feasibility of using MAH1 for the bioremediation of BTEX-contaminated sites.
    Materialart: Online-Ressource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2015
    ZDB Id: 280534-0
    ZDB Id: 1481972-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2014
    In:  Canadian Journal of Microbiology Vol. 60, No. 3 ( 2014-03), p. 167-171
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 60, No. 3 ( 2014-03), p. 167-171
    Kurzfassung: To expand current knowledge on the molecular aspects of alkaline phosphatase PhoX in shallow eutrophic freshwaters, we investigated the genetic diversity and abundance of the PhoX-encoding gene (phoX) in 4 ecological regions in Lake Taihu, China, following a gradient in total phosphorus concentrations ranging from hypereutrophic to mesotrophic. Bacterial phoX was heterogeneously distributed with the highest diversity in the eutrophic regions and the highest abundance in the mesotrophic Xukou Bay. The concentrations of total phosphorus and enzymatically hydrolyzable phosphorus determined the distribution of bacterial phoX in Lake Taihu. Most (70.8%) of the phoX-translated proteins had 〈 90% similarity to the PhoX proteins in the GenBank database, suggesting the presence of novel phoX genotypes in Lake Taihu. The low overlap in phoX genotypes (15.8%) between Lake Taihu and some marine ecosystems, and the dominance of the translated proteins most similar to the Alphaproteobacteria-affiliated PhoX, demonstrate the uniqueness of PhoX in eutrophic freshwaters.
    Materialart: Online-Ressource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2014
    ZDB Id: 280534-0
    ZDB Id: 1481972-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    Canadian Science Publishing ; 2015
    In:  Canadian Journal of Microbiology Vol. 61, No. 3 ( 2015-03), p. 227-236
    In: Canadian Journal of Microbiology, Canadian Science Publishing, Vol. 61, No. 3 ( 2015-03), p. 227-236
    Kurzfassung: Limnetic habitats that are dominated by either algae or macrophytes represent the 2 dominant ecosystems in shallow lakes. We assessed seasonal variations in the diversity and abundance of alkaline phosphate-encoding genes (phoX) in these 2 zones of Lake Taihu, which is a large, shallow, eutrophic lake in China. There was no significant difference in seasonal mean phoX diversity between the 2 zones, whereas the seasonal mean phoX abundance in the macrophyte-dominated region was higher than that in the algae-dominated region. The bulk of the genotypes in the 2 regions were most similar to the alphaproteobacterial and betaproteobacterial phoX. Genotypes most similar to phoX affiliated with Betaproteobacteria were present with greater diversity in the macrophyte-dominated zone than in the algae-dominated zone. In the algae-dominated zone, the relative proportion of genotypes most similar to cyanobacterial phoX was highest (38.8%) in summer. In addition to the different genotype structures and environmental factors between the 2 stable states, the lower gene abundances and higher alkaline phosphatase activities in Meiliang Bay in summer than those in Xukou Bay reveals different organophosphate-mineralizing modes in these 2 contrasting habitats.
    Materialart: Online-Ressource
    ISSN: 0008-4166 , 1480-3275
    RVK:
    Sprache: Englisch
    Verlag: Canadian Science Publishing
    Publikationsdatum: 2015
    ZDB Id: 280534-0
    ZDB Id: 1481972-7
    SSG: 12
    Standort Signatur Einschränkungen Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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