In:
Central Nervous System Agents in Medicinal Chemistry, Bentham Science Publishers Ltd., Vol. 22, No. 3 ( 2022-12), p. 175-187
Abstract:
Ischemic stroke remains the leading cause of death worldwide and is the
primary cause of disability globally. Numerous studies have shown that plant-origin medicines are promising and can influence the treatment of neurological disorders. Phyllanthus embilica L. (P.
emblica or Amla) is one of the herbal plants whose medicinal properties are widely studied. The objective of the present study is to determine the neuroprotective effects of an aqueous extract of
the fruit of P. emblica (hereinafter referred to as just P. emblica) on cerebral ischemia-reperfusion injury and explore if it can regulate BDNF/PI3K pathway to modulate glutathione for mitoprotection
and neuroprotection. Methods: In vivo studies were conducted on male Sprague Dawley rats, where rats were prophylactically
administered 100 mg/kg P. emblica for 30 days. In the treatment group, rats were given 100 mg/kg P. emblica, 1 h post middle cerebral artery occlusion (MCAo). Rats were evaluated for
neuro deficit and motor function tests. Brains were further harvested for infarct size evaluation, biochemical analysis, protein expression studies, and mitochondrial studies. Results: Prophylaxis and treatment with P. emblica demonstrated significant improvement in
functional outcome with a reduction in infarct size. Normalization of glutathione, nitrite, and malondialdehyde levels was also observed. Improvement in mitochondrial complex I and IV activities
was also reported. Expressions of BDNF, PI3K, SDF1 and VEGF increased while that of ROCK2 decreased following P. emblica administration. Conclusion: P. emblica regulates BDNF/PI3K pathway to modulate glutathione in ischemic
stroke to confer mitoprotection and neuroprotection.
Type of Medium:
Online Resource
ISSN:
1871-5249
DOI:
10.2174/1871524922666220607093400
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2022
SSG:
15,3
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