In:
Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 45, No. 9 ( 2001-09), p. 2495-2501
Abstract:
Prolonged treatment of chronic hepatitis B virus (HBV) infection with lamivudine ([−]-β- l -2′,3′-dideoxy-3′ thiacytidine) or famciclovir may select for viral mutants that are drug resistant due to point mutations in the polymerase gene. Determining whether such HBV mutants are sensitive to new antiviral agents is therefore important. We used a transient transfection system to compare the sensitivities of wild-type HBV and four lamivudine- and/or famciclovir-resistant HBV mutants to adefovir [9-(2-phosphonyl-methoxyethyl)-adenine; PMEA] and the nucleoside analogues (−)-β- d -2, 6-diaminopurine dioxolane (DAPD) and 2′-fluoro-5-methyl-β- l -arabinofuranosyluracil ( l -FMAU). The drug-resistant mutants contained amino acid substitutions in the polymerase protein. We found that the M550I and M550V plus L526M substitutions, which confer lamivudine resistance, did not confer cross-resistance to adefovir or DAPD, but conferred cross-resistance to l -FMAU. The M550V substitution in isolation conferred a similar phenotype to M550I, except that it did not confer significant resistance to l -FMAU. The L526M substitution, which is associated with famciclovir resistance, conferred cross-resistance to l -FMAU but not to adefovir or DAPD. Inhibition of HBV secretion by DAPD, l -FMAU, and adefovir did not always correlate with inhibition of the generation of intracellular HBV replicative intermediates, suggesting that these analogs may preferentially inhibit specific stages of the viral replication cycle.
Type of Medium:
Online Resource
ISSN:
0066-4804
,
1098-6596
DOI:
10.1128/AAC.45.9.2495-2501.2001
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2001
detail.hit.zdb_id:
1496156-8
SSG:
12
SSG:
15,3
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