In:
TECHNOLOGY, World Scientific Pub Co Pte Ltd, Vol. 02, No. 03 ( 2014-09), p. 243-253
Abstract:
Decellularization of whole organs, such as the kidney hold great promise in addressing donor shortage for transplantation. However, successful implantation of engineered whole kidney constructs has been challenged by the inability to maintain endothelial cell coverage of the vasculature matrix, resulting in excessive blood clots, loss of vascular patency, and cell death within the construct. In this study, we describe an endothelial cell seeding approach that permits effective coating of the vascular matrix of the decellularized porcine kidney scaffold using a combination of static and ramping perfusion cell seeding. Furthermore, conjugation of CD31 antibodies to the vascular matrix improved endothelial cell retention on the vasculatures, which enhanced vascular patency of the implanted scaffold. These results demonstrate that our endothelial cell seeding method combined with antibody conjugation improves endothelial cell attachment and retention leading to vascular patency of tissue-engineered whole kidney in vivo.
Type of Medium:
Online Resource
ISSN:
2339-5478
,
2345-7740
DOI:
10.1142/S2339547814500228
Language:
English
Publisher:
World Scientific Pub Co Pte Ltd
Publication Date:
2014
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