In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 63, No. Pt_6 ( 2013-06-01), p. 2254-2259
Abstract:
A novel Gram-positive, multiloculated thalli-forming, aerobic, actinobacterial strain, CF9/1/1 T , was isolated in 2007 during environmental screening for xerophilic fungi in arid desert soil from the Sahara desert, Chad. The isolate grew best at a temperature range of 20–35 °C and at pH 6.0–8.5 and with 0–4 % (w/v) NaCl, forming black-coloured and irregular colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus . The DNA G+C content of the novel strain was 75.4 mol%. The peptidoglycan contained meso -diaminopimelic acid as a diagnostic diamino acid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, a not yet structurally identified aminophospholipid and a small amount of phosphatidylglycerol; MK-9(H 4 ) was identified as the dominant menaquinone and galactose was a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C 16 : 0 and iso-C 15 : 0 . The 16S rRNA gene sequence of the isolate showed 94.6–97.0 % sequence similarities with those of five members of the genus: Geodermatophilus ruber DSM 45317 T (94.6 %), Geodermatophilus obscurus DSM 43160 T (94.8 %), Geodermatophilus siccatus DSM 45419 T (96.2 %), Geodermatophilus nigrescens DSM 45408 T (96.7 %) and Geodermatophilus arenarius DSM 45418 T (97.0 %). Based on the evidence from this polyphasic taxonomic study, a novel species, Geodermatophilus telluris sp. nov., is proposed; the type strain is CF9/1/1 T ( = DSM 45421 T = CCUG 62764 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.046888-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2013
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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