In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 71, No. 4 ( 2021-04-13)
Abstract:
A novel Gram-stain-negative, facultative aerobic and rod-shaped bacterium, designated as MKL-01 T and isolated from the blood of immunocompromised patient, was genotypically and phenotypically characterized. The colonies were found to be creamy yellow and convex. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that strain MKL-01 T was most closely related to Cupriavidus gilardii LMG 5886 T , present within a large cluster in the genus Cupriavidus . The genome sequence of strain MKL-01 T showed the highest average nucleotide identity value of 92.1 % and digital DNA–DNA hybridization value of 44.8 % with the closely related species C. gilardii LMG 5886 T . The genome size of the isolate was 5 750 268 bp, with a G+C content of 67.87 mol%. The strain could grow at 10–45 °C (optimum, 37–40 °C), in the presence of 0–10 % (w/v) NaCl (optimum, 0.5%) and at pH 6.0–10.0 (optimum, pH 7.0). Strain MKL-01 T was positive for catalase and negative for oxidase. The major fatty acids were C 16 : 0 , summed feature 3 (C 16 : 1 ω 7 c /C 16 : 1 ω 6 c and/or C 16 : 1 ω 6 c /C 16 : 1 ω 7 c ) and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and one unidentified polar lipid. Moreover, strain MKL-01 T contained ubiquinone Q-8 as the sole respiratory quinone. Based on its molecular, phenotypic and chemotaxonomic properties, strain MKL-01 T represents a novel species of the genus Cupriavidus ; the name Cupriavidus cauae sp. nov. is proposed for this strain. The type strain is MKL-01 T .
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.004759
Language:
English
Publisher:
Microbiology Society
Publication Date:
2021
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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