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  • Oxford University Press (OUP)  (4)
  • Li, Ran  (4)
  • English  (4)
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  • Oxford University Press (OUP)  (4)
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  • English  (4)
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  • 1
    Online Resource
    Online Resource
    Comprehensive Analysis of Volatile Compounds in Mouthpiece Cigarette Adhesive by Coupling Headspace with Gas Chromatography–Mass Spectrometry
    Oxford University Press (OUP) ; 2021
    In:  Journal of AOAC INTERNATIONAL Vol. 104, No. 3 ( 2021-06-12), p. 712-718
    Details
    In: Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 104, No. 3 ( 2021-06-12), p. 712-718
    Abstract: Some volatile compounds in mouthpiece cigarette adhesive emit irritating odors and affect the taste of smoking cigarettes. Therefore, it is necessary to monitor the volatile compounds in mouthpiece cigarette adhesive. Objectives A rapid and simple analytical method of volatile compounds in mouthpiece cigarette adhesive was established. Methods In this study, headspace (HS) injection coupled with GC–MS was utilized for qualitative and quantitative analysis. Initially, the volatile compounds in mouthpiece cigarette adhesives were detected by HS-GC–MS, followed by spectrum library retrieval. The detected compounds with the similarity to a spectrum library of more than 85% were further identified by comparing the retention time and mass spectra of the detected volatile compounds and those of the corresponding standard samples. The quantitative analysis of nine identified volatile compounds was performed. Results Eleven volatile compounds in the mouthpiece cigarette adhesive were accurately identified. The quantitative analytical method of nine volatile compounds in mouthpiece cigarette adhesive was validated to have good linearities (R2 & gt; 0.9932) within the range of 20–5000 ng/g. The detection limits of 9 compounds were within the range of 3.1–147.7 ng/g. The intra- and inter-day relative standard deviations were less than 19.8%. The recoveries of these 9 compounds spiked into mouthpiece cigarette adhesive were 68.1–108.3%. Conclusions The proposed method is rapid, simple, and accurate for qualitative and quantitative analysis of volatile compounds in the mouthpiece cigarette adhesive. Highlights The developed analytical method is expected to be used to monitor volatile compounds in various adhesives.
    Type of Medium: Online Resource
    ISSN: 1060-3271 , 1944-7922
    URL: Article
    DOI: 10.1093/jaoacint/qsaa160
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Molecular phylogeny and taxonomy of three anaerobic plagiopyleans (Alveolata: Ciliophora), retrieved from two geographically distant localities in Asia and North America
    Oxford University Press (OUP) ; 2023
    In:  Zoological Journal of the Linnean Society Vol. 199, No. 2 ( 2023-10-03), p. 493-510
    Details
    In: Zoological Journal of the Linnean Society, Oxford University Press (OUP), Vol. 199, No. 2 ( 2023-10-03), p. 493-510
    Abstract: Anaerobic ciliates are key organisms for studying adaptations to an anaerobic lifestyle, the evolution of eukaryotic cell organelles, mechanisms of energy metabolism, and symbiosis with prokaryotes; however, the biodiversity of their marine representatives remains poorly known. In this study, three species of ciliates belonging to the class Plagiopylea, i.e. Trimyema foissnerisp. nov.,Plagiopyla rarisetasp. nov., and Plagiopyla frontata, collected from various marine anoxic habitats from two geographically distant continents, Asia and North America, were investigated using morphological and molecular methods. Trimyema foissneri can be recognized by three complete somatic ciliary girdles, special arrangement of kinetosomes near the anterior end of the oral kineties, and the presence of a ventrolateral fragment. Plagiopyla rariseta is distinguished by sparse cilia on the frontal region and particular orientation of the buccal cavity. Plagiopyla frontata is newly recorded and is redescribed in detail and redefined based on new populations. Phylogenetic analyses based on the small subunit ribosomal ribonucleic acid (SSU rRNA) gene supported the generic assignment of the three species and corroborated the monophyly of the families Trimyemidae and Plagiopylidae. An improved key to the identification of the valid species of genus Trimyema is also supplied.
    Type of Medium: Online Resource
    ISSN: 0024-4082 , 1096-3642
    URL: Article
    DOI: 10.1093/zoolinnean/zlad015
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2023
    detail.hit.zdb_id: 1471951-4
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Swimming performance of a pelagic species in the Yangtze River under different exposure modes of the total dissolved gas supersaturation
    Oxford University Press (OUP) ; 2022
    In:  Conservation Physiology Vol. 10, No. 1 ( 2022-01-01)
    Details
    In: Conservation Physiology, Oxford University Press (OUP), Vol. 10, No. 1 ( 2022-01-01)
    Abstract: During flood discharges of upstream dams in the Yangtze River, the pelagic fish have a stress risk from total dissolved gas (TDG) supersaturation in the river water. This study took the silver carp as the object and systematically evaluated the effects of TDG supersaturation levels and exposure time on their critical swimming speed (Ucrit) at different temperatures. The external symptoms of gas bubble disease were found when TDG levels exceeded 130%. Both exposure time and TDG level did not significantly impact the Ucrit of fish under 6 days of non-lethal exposure (110%, 120%, 130% TDG) with lower or higher water temperature. Significant differences in Ucrit were found among different exposure times at 11.0 ± 1.0°C under 10 hours of lethal exposure (135%, 140%, 150% TDG) and the Ucrit reduced by 59.88%, 83.32%, and 92.40%, respectively. TDG level had a significant impact on the Ucrit at 21.0 ± 1.0°C when exposure time exceeded 8 hours. Ucrit at 21.0 ± 1.0°C water were significantly greater than those at 11.0 ± 1.0°C water where conditions had the same TDG supersaturation and exposure time. Differences in Ucrit between temperatures ranged from 3.24 to 6.12 BL/s under non-lethal exposure and from 6.38 to 13.88 BL/s under lethal exposure. The results of this study can provide a reference for fish conservation during flood discharge.
    Type of Medium: Online Resource
    ISSN: 2051-1434
    URL: Article
    DOI: 10.1093/conphys/coac047
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 2721508-8
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  • 4
    Online Resource
    Online Resource
    Cytotoxic function of xylanase VdXyn4 in the plant vascular wilt pathogen Verticillium dahliae
    Oxford University Press (OUP) ; 2021
    In:  Plant Physiology Vol. 187, No. 1 ( 2021-09-04), p. 409-429
    Details
    In: Plant Physiology, Oxford University Press (OUP), Vol. 187, No. 1 ( 2021-09-04), p. 409-429
    Abstract: Phytopathogen xylanases play critical roles in pathogenesis, likely due to their ability to degrade plant structural barriers and manipulate host immunity. As an invader of plant xylem vessels, the fungus Verticillium dahliae is thought to deploy complex cell wall degrading enzymes. Comparative genomics analyses revealed that the V. dahliae genome encodes a family of six xylanases, each possessing a glycosyl hydrolase 11 domain, but the functions of these enzymes are undetermined. Characterizing gene deletion mutants revealed that only V. dahliae xylanase 4 (VdXyn4) degraded the plant cell wall and contributed to the virulence of V. dahliae. VdXyn4 displayed cytotoxic activity and induced a necrosis phenotype during the late stages of infection, leading to vein and petiole collapse that depended on the enzyme simultaneously localizing to nuclei and chloroplasts. The internalization of VdXyn4 was in conjunction with that of the plasma membrane complexLeucine-rich repeat (LRR)-receptor-like kinase suppressor of BIR1-1 (SOBIR1)/LRR-RLK BRI1-associated kinase-1 (BAK1), but we could not rule out the possibility that VdXyn4 may also act as an apoplastic effector. Immune signaling (in the SA–JA pathways) induced by VdXyn4 relative to that induced by known immunity effectors was substantially delayed. While cytotoxic activity could be partially suppressed by known effectors, they failed to impede necrosis in Nicotiana benthamiana. Thus, unlike typical effectors, cytotoxicity of VdXyn4 plays a crucial intracellular role at the late stages of V. dahliae infection and colonization, especially following pathogen entry into the xylem; this cytotoxic activity is likely conserved in the corresponding enzyme families in plant vascular pathogens.
    Type of Medium: Online Resource
    ISSN: 0032-0889 , 1532-2548
    URL: Article
    DOI: 10.1093/plphys/kiab274
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
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