In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 5 ( 2022-05-17)
Abstract:
A Gram-stain-positive coccus was isolated from the blood of a paediatric patient suffering from gastroenteritis. The taxonomic position of this catalase-positive, non-motile, non-spore-forming facultative anaerobe designated as strain MKL-02 T was investigated using a polyphasic approach. Colonies grown on tryptic soy agar with 10 % sheep blood were circular, creamy yellow, and convex. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that this strain was most closely related to Arsenicicoccus bolidensis CCUG 47306 T within the cluster of the genus Arsenicicoccus . Average nucleotide identity and digital DNA–DNA hybridization values between strain MKL-02 T and A. bolidensis DSM 15745 T , A. dermatophillus DSM 25571 T and A. piscis DSM 22760 T were 89.5 and 37.0 %, 79.6 and 22.4 %, and 75.9 and 21.0 %, respectively. The genomic size of strain MKL-02 T was 3 423 857 bp with a 72.7 mol% G+C content. Growth was observed at 10–45 °C (optimum, 37–40 °C) and pH 6.0–10.0 (optimum, pH 7.0), in the presence of 0–10 % (w/v) NaCl (optimum, 0.5 %). Cells of strain MKL-02 T were non-motile cocci and 0.50–0.60 µm long, as determined by transmission electron microscopy. The strain was catalase-positive and oxidase-negative. The major fatty acid type ( 〉 10 % of total) was C 15 : 0 . The polar lipid profile consisted of two unidentified phospholipids, three unidentified lipids and an unidentified aminophospholipid. The strain contained MK-8 (H 4 ) as the predominant menaquinone. Based on phylogenetic and phenotypic considerations, it is proposed that strain MKL-02 T be classified as a new species, named Arsenicicoccus cauae sp. nov. The type strain is MKL-02 T (=NCCP 16967 T =JCM 34624 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005381
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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