In:
Frontiers in Immunology, Frontiers Media SA, Vol. 11 ( 2021-1-22)
Abstract:
This study aimed to characterize the tumor-infiltrating T cells in moderately differentiated colorectal cancer. Methods Using single-cell RNA sequencing data of isolated 1632 T cells from tumor tissue and 1252 T cells from the peripheral blood of CRC patients, unsupervised clustering analysis was performed to identify functionally distinct T cell populations, followed by correlations and ligand-receptor interactions across cell types. Finally, differential analysis of the tumor-infiltrating T cells between colon cancer and rectal cancer were carried out. Results A total of eight distinct T cell populations were identified from tumor tissue. Tumor-Treg showed a strong correlation with Th17 cells. CD8 + T RM was positively correlated with CD8 + IEL. Seven distinct T cell populations were identified from peripheral blood. There was a strong correlation between CD4+T N and CD4+blood-T CM . Colon cancer and rectal cancer showed differences in the composition of tumor-infiltrating T cell populations. Tumor-infiltrating CD8 + IEL cells were found in rectal cancer but not in colon cancer, while CD8 + T N cells were found in the peripheral blood of colon cancer but not in that of rectal cancer. A larger number of tumor-infiltrating CD8 + Tex (88.94%) cells were found in the colon cancer than in the rectal cancer (11.06%). The T cells of the colon and rectal cancers showed changes in gene expression pattern. Conclusions We characterized the T cell populations in the CRC tumor tissue and peripheral blood.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2020.620196
DOI:
10.3389/fimmu.2020.620196.s001
DOI:
10.3389/fimmu.2020.620196.s002
DOI:
10.3389/fimmu.2020.620196.s003
DOI:
10.3389/fimmu.2020.620196.s004
DOI:
10.3389/fimmu.2020.620196.s005
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2606827-8
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