In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-11-23)
Abstract:
Klebsiella pneumoniae is a primary culprit of antibiotic-resistant nosocomial infections worldwide, and infections caused by NDM-producing strains are a major threat due to limited therapeutic options. The majority of bla NDM cases occur on plasmids; therefore, we explored the relationships between plasmids and bla NDM genes in K. pneumoniae by analyzing the variants of bla NDM , replicon types, conjugative transfer regions of 171 bla NDM -harboring plasmids from 4,451 K. pneumoniae plasmids. Of the nine identified bla NDM variants, bla NDM-1 (73.68%) and bla NDM-5 (16.37%) were the most dominant. Over half of the bla NDM -harboring plasmids of K. pneumoniae were classified into IncF plasmids. IncX3 single-replicon plasmids (46–57 kb) carried genes encoding relaxases of the MOB P family, T4CP genes of the VirD4/TraG subfamily, and VirB-like T4SS gene clusters, which were mainly geographically distributed in China. We found 10 bla NDM -harboring IncN plasmids (38.38–63.05 kb) carrying the NW-type origin of transfer ( oriT ) regions, genes coding for relaxases of MOB F family, genes encoding T4CPs of the TrwB/TraD subfamily, and Trw-like T4SS gene clusters, which were also mainly geographically distributed in China. Moreover, we identified 21 IncC plasmids carrying bla NDM-1 (140.1–329.2 kb), containing the A/C-type oriT s, genes encoding relaxases of MOB H family, genes encoding T4CPs belonging to TrwB/TraD subfamily, and Tra_F-like T4SS gene clusters. The bla NDM -harboring IncC plasmids were widely geographically distributed all over the world, mainly in the United States, China and Viet Nam. These findings enhance our understanding of the diversity of bla NDM -harboring plasmids in K. pneumoniae .
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2022.1008905
DOI:
10.3389/fmicb.2022.1008905.s001
DOI:
10.3389/fmicb.2022.1008905.s002
DOI:
10.3389/fmicb.2022.1008905.s003
DOI:
10.3389/fmicb.2022.1008905.s004
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2587354-4
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