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  • 1
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    Online Resource
    Intestine epithelial cell-Derived Extracellular Vesicles alleviate Inflammation Induced by Clostridioides Difficile TcdB through the Activity of TGF- β1
    Auctores Publishing LLC ; 2021
    In:  Immunology and Inflammation Diseases Therapy Vol. 4, No. 1 ( 2021-11-30), p. 01-09
    Details
    In: Immunology and Inflammation Diseases Therapy, Auctores Publishing LLC, Vol. 4, No. 1 ( 2021-11-30), p. 01-09
    Abstract: Background: Clostridioides difficile infection (CDI) has been primarily associated with the toxin B (TcdB), which can activate the intestinal immune system and lead to pathological damage. Even though the biological functions of intestine epithelial cell- derived extracellular vesicles (I-Evs) have been well documented, the role of I-Evs in the process of CDI is still unknown. Results: We isolated I-Evs ranging from 100–200 nm in mean diameter, with a density of 1.09-1.17 g/mL. These I-Evs expressed the extracellular vesicle-associated specific surface markers, CD63 and TSG101. In vitro, 50 µg I-Evs decreased the expression of IL-6, TNF- β, IL-1β, and IL-22 in MC38 induced by 0.8 ng/mL C. difficile TcdB, and increased expression of TGF- β1. In vivo, I-Evs also promoted regulatory T cell induction, which improved inflammation of mice up to 80% relative to C. difficile TcdB infected mice, depending on the TGF- β1 signal pathway. Conclusion: Our study firstly demonstrated that I-Evs originated from intestine epithelial cells is potentially a novel treatment endogenous candidate to effectively reduce the local infection induced by C. difficile TcdB.
    Type of Medium: Online Resource
    ISSN: 2637-8876
    Uniform Title: Intestine epithelial cell-Derived Extracellular Vesicles alleviate Inflammation Induced by Clostridioides Difficile TcdB through the Activity of TGF- β1
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.31579/2637-8876/01.2021
    DOI: 10.31579/2637-8876/JIIDT/
    DOI: 10.31579/2637-8876/018
    Language: Unknown
    Publisher: Auctores Publishing LLC
    Publication Date: 2021
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  • 2
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    Simulation and Energy-Saving Algorithm Based on MRAC of VAV BOX
    Trans Tech Publications, Ltd. ; 2013
    In:  Advanced Materials Research Vol. 732-733 ( 2013-8), p. 615-619
    Details
    In: Advanced Materials Research, Trans Tech Publications, Ltd., Vol. 732-733 ( 2013-8), p. 615-619
    Abstract: A model reference adaptive control (MRAC) algorithm is proposed to the air-conditioning system of variable air volume (VAV) terminal units (VAV BOX). The study of temperature controlling parameters in room area (Zone) is based on Lyapunov stability theory. We built the simulation model in Matlab to validate the superior controlling performance and good energy saving effect of the algorithm. The research shows that the energy consumption release 60% ~70% in larger room. In modern building, the energy saving and comfort is very important, so the simulation has important engineering practical value.
    Type of Medium: Online Resource
    ISSN: 1662-8985
    URL: Issue
    URL: Article
    DOI: 10.4028/www.scientific.net/AMR.732-733
    DOI: 10.4028/www.scientific.net/AMR.732-733.615
    Language: Unknown
    Publisher: Trans Tech Publications, Ltd.
    Publication Date: 2013
    detail.hit.zdb_id: 2265002-7
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  • 3
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    Right ventricular systolic function and associated anatomic risk factors in fetuses with transposition of the great arteries: Evaluation by velocity vector imaging
    Frontiers Media SA ; 2023
    In:  Frontiers in Cardiovascular Medicine Vol. 9 ( 2023-1-10)
    Details
    In: Frontiers in Cardiovascular Medicine, Frontiers Media SA, Vol. 9 ( 2023-1-10)
    Abstract: The aim of this study was to evaluate right ventricular (RV) systolic function in fetuses with transposition of the great arteries (TGA) using velocity vector imaging (VVI) and to investigate the impact of different factors on RV systolic function in TGA fetuses. Methods This was a retrospective cross-sectional study of fetuses referred to our tertiary center between 2015 and 2019. Maternal and fetal baseline characteristics and conventional echocardiographic and myocardial deformation indices were collected in fetuses with TGA at 20–28 weeks’ gestation, which were compared with normal fetuses with comparable gestational age (GA). RV deformational parameters including global and regional longitudinal peak systolic strain, strain rate, and velocity were measured using off-line speckle tracking analysis. The univariate and multivariate linear regression analyses were established to evaluate the independent risk factors for RV global longitudinal systolic strain (RVGLSs) and strain rate (RVGLSRs). Results In total, 78 fetuses with TGA [including 49 fetuses with complete transposition of the great arteries (d-TGA) and 29 fetuses with Taussig-Bing anomaly (TBA)] and 49 normal fetuses were included. Compared with normal controls, global and most regional RV longitudinal systolic peak velocity, strain, and strain rate were lower in d-TGA and TBA fetuses ( P & lt; 0.05). Compared with normal controls, global and most regional RV longitudinal systolic strain was lower in d-TGA fetuses without pulmonary stenosis (PS) and ventricular septal defect (VSD), while RVGLSs and RVGLSRs were lower in TBA fetuses without PS. The VSD was an independent determinant of RVGLSRs ( P = 0.024) in the d-TGA group. Additionally, PS was an independent determinant of RVGLSs and RVGLSRs ( P = 0.012, P = 0.027) in the TBA group. Conclusion Early impairment of RV systolic function has already occurred in TGA fetuses during the 2nd trimester of pregnancy. PS, VSD, and foramen ovale (FO) were independent risk factors for decreased RV function.
    Type of Medium: Online Resource
    ISSN: 2297-055X
    URL: Article
    DOI: 10.3389/fcvm.2022.973395
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2781496-8
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  • 4
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    Data_Sheet_5.PDF
    Frontiers Media SA ; 2024
    In:  Frontiers in Microbiology Vol. 15 ( 2024-4-4)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 15 ( 2024-4-4)
    Abstract: Clostridioides difficile infection (CDI), as well as its etiology and pathogenesis, have been extensively investigated. However, the absence of suitable CDI animal models that reflect CDI symptoms and the associated gut microbiome changes in humans has limited research progress in this field. Thus, we aimed to investigate whether Mongolian gerbils, which present a range of human pathological conditions, can been used in studies on CDI. Methods: In this study, we infected Mongolian gerbils and two existing CDI model animals, mice and hamsters, with the hypervirulent ribotype 027 C. difficile strain, and comparatively analyzed changes in their gut microbiome composition via 16S rRNA gene sequencing. Methods In this study, we infected Mongolian gerbils and two existing CDI model animals, mice and hamsters, with the hypervirulent ribotype 027 C. difficile strain, and comparatively analyzed changes in their gut microbiome composition via 16S rRNA gene sequencing. Results The results obtained showed that C. difficile colonized the gastrointestinal tracts of the three rodents, and after the C. difficile challenge, C57BL/6J mice did not manifest CDI symptoms and their intestines showed no significant pathological changes. However, the hamsters showed explosive intestinal bleeding and inflammation and the Mongolian gerbils presented diarrhea as well as increased infiltration of inflammatory cells, mucus secretion, and epithelial cell shedding in their intestinal tissue. Further, intestinal microbiome analysis revealed significant differences with respect to intestinal flora abundance and diversity. Specifically, after C. difficile challenge, the Firmicutes/Bacteroidetes ratio decreased for C57BL/6J mice, but increased significantly for Mongolian gerbils and hamsters. Furthermore, the abundance of Proteobacteria increased in all three models, especially in hamsters, while that of Verrucomicrobia only increased significantly in C57BL/6J mice and Mongolian gerbils. Our results also indicated that differences in the relative abundances of Lactobacillaceae and Akkermansia were primarily responsible for the observed differences in response to C. difficile challenge. Conclusion Based on the observed responses to C. difficile challenge, we concluded for the first time that the Mongolian gerbil could be used as an animal model for CDI. Additionally, the taxa identified in this study may be used as biomarkers for further studies on CDI and to improve understanding regarding changes in gut microbiome in CDI-related diseases.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2024.1368194
    DOI: 10.3389/fmicb.2024.1368194.s001
    DOI: 10.3389/fmicb.2024.1368194.s002
    DOI: 10.3389/fmicb.2024.1368194.s003
    DOI: 10.3389/fmicb.2024.1368194.s004
    DOI: 10.3389/fmicb.2024.1368194.s005
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2024
    detail.hit.zdb_id: 2587354-4
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  • 5
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    DataSheet1.docx
    Frontiers Media SA ; 2022
    In:  Frontiers in Bioengineering and Biotechnology Vol. 10 ( 2022-5-19)
    Details
    In: Frontiers in Bioengineering and Biotechnology, Frontiers Media SA, Vol. 10 ( 2022-5-19)
    Abstract: Ultrafast, portable, and inexpensive molecular diagnostic platforms are critical for clinical diagnosis and on-site detection. There are currently no available real-time polymerase chain reaction (PCR) devices able to meet the demands of point-of-care testing, as the heating and cooling processes cannot be avoided. In this study, the dual temperature modules were first designed to process microfluidic chips automatically circulating between them. Thus, a novel ultrafast molecular diagnostic real-time PCR device (approximately 18 and 23 min for DNA and RNA detection, respectively) with two channels (FAM and Cy5) for the detection of 12 targets was developed. The device contained three core functional components, including temperature control, optics, and motion, which were integrated into a portable compact box. The temperature modules accurately control temperature in rapid thermal cycles with less than ±0.1 °C, ±1 °C and ±0.5 °C for the temperature fluctuation, uniformity, and error of indication, respectively. The average coefficient of variation (CV) of the fluorescence intensity (FI) for all 12 wells was 2.3% for FAM and 2.7% for Cy5. There was a good linear relationship between the concentrations of fluorescent dye and the FIs of FAM and Cy5( R 2 = 0.9990 and 0.9937), and the average CVs of the Ct values calculated by the embedded software were 1.4% for FAM and Cy5, respectively. The 100 double-blind mocked sputum and 249 clinical stool samples were analyzed by the ultrafast real-time PCR device in comparison with the DAAN Gene SARS-CoV-2 kit run on the ABI 7500 instrument and Xpert C. difficile /Epi, respectively. Among the 249 stool samples, the ultrafast real-time PCR device detected toxigenic C. difficile in 54 samples (54/249, 21.7%) with a specificity and positive predictive values of 99.0 and 96.3%, which were higher than the Xpert C. difficile /Epi values of 94.4 and 88.1% ( p & gt; 0.05). The ultrafast real-time PCR device detected 15 SARS-CoV-2 positive samples, which has a 100% concordance with that obtained by the DAAN Gene SARS-CoV-2 kit. This study demonstrated that the ultrafast real-time PCR device integrated with microfluidic chips and dual temperature modules is an ultrafast, reliable, easy-to-use, and cost-effective molecular diagnostic platform for clinical diagnosis and on-site testing, especially in resource-limited settings.
    Type of Medium: Online Resource
    ISSN: 2296-4185
    URL: Article
    URL: Article
    DOI: 10.3389/fbioe.2022.895236
    DOI: 10.3389/fbioe.2022.895236.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2719493-0
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  • 6
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    Table_1.XLSX
    Frontiers Media SA ; 2021
    In:  Frontiers in Microbiology Vol. 12 ( 2021-7-5)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 12 ( 2021-7-5)
    Abstract: Clostridioides difficile sequence type 2 (ST2) has been increasingly recognized as one of the major genotypes in China, while the genomic characteristics and biological phenotypes of Chinese ST2 strains remain to be determined. We used whole-genome sequencing and phylogenetic analysis to investigate the genomic features of 182 ST2 strains, isolated between 2011 and 2017. PCR ribotyping (RT) was performed, and antibiotic resistance, toxin concentration, and sporulation capacity were measured. The core genome Maximum-likelihood phylogenetic analysis showed that ST2 strains were distinctly segregated into two genetically diverse lineages [L1 (67.0% from Northern America) and L2], while L2 further divided into two sub-lineages, SL2a and SL2b (73.5% from China). The 36 virulence-related genes were widely distributed in ST2 genomes, but in which only 11 antibiotic resistance-associated genes were dispersedly found. Among the 25 SL2b sequenced isolates, RT014 (40.0%, n = 10) and RT020 (28.0%, n = 7) were two main genotypes with no significant difference on antibiotic resistance (χ 2 = 0.024–2.667, P & gt; 0.05). A non-synonymous amino acid substitution was found in tcdB (Y1975D) which was specific to SL2b. Although there was no significant difference in sporulation capacity between the two lineages, the average toxin B concentration (5.11 ± 3.20 ng/μL) in SL2b was significantly lower in comparison to those in L1 (10.49 ± 15.82 ng/μL) and SL2a (13.92 ± 2.39 ng/μL) (χ 2 = 12.30, P & lt; 0.05). This study described the genomic characteristics of C. difficile ST2, with many virulence loci and few antibiotic resistance elements. The Chinese ST2 strains with the mutation in codon 1975 of the tcdB gene clustering in SL2b circulating in China express low toxin B, which may be associated with mild or moderate C. difficile infection.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2021.651520
    DOI: 10.3389/fmicb.2021.651520.s001
    DOI: 10.3389/fmicb.2021.651520.s002
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2587354-4
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  • 7
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    Table_1.DOCX
    Frontiers Media SA ; 2021
    In:  Frontiers in Marine Science Vol. 8 ( 2021-10-26)
    Details
    In: Frontiers in Marine Science, Frontiers Media SA, Vol. 8 ( 2021-10-26)
    Abstract: High trophic plasticity is often invoked to explain the successful expansion of many aquatic species. Bombay duck (BD) outcompete other traditionally commercial fishes in the East China Sea (ECS) in recent years, displaying a continuous and sharp increase of catches, however, little is known whether the competition advantage of BD was related to its trophic plasticity. Using stable isotope analysis (SIA), we investigated the trophic ecology of BD in the south-central ECS in two periods. Significant differences in δ 13 C and δ 15 N values were found between sampling months and among body sizes. Modal (95% credibility limits) estimation showed that BD’s trophic position (TP) gradually increased from 3.56 (3.31–3.82) in G1 (120–150 mm for body size) to 4.09 (3.69–4.50) in G5 (241–270 mm) in November, and from 3.37 (2.92–3.88) in G2 (151–180 mm) to 3.66 (3.07–4.23) in G4 (211–240 mm) in April, respectively, suggesting obvious ontogenetic variation of δ 15 N and TP. More importantly, the TP of BD within the same body size showed wide amplitude, indicating BD possess high trophic plasticity, and can capture prey from low to high trophic levels. Further quantitative analysis based on the MixSIAR model showed that BD mainly fed on zooplankton and fish, but its feeding habit was characterized by the consumption of gradually reduced zooplankton and increased fish associated with increased body size. Interestingly, the fish’s contribution to BD showed obvious seasonal variation. It can be explained by varied food availability in ECS. Relatively low fish prey in April forced BD to adjust its diet, adding the ingestion of shrimps and cephalopods corresponding to temporal variations in food availability. The present study provides the evidence that high trophic plasticity exists in the feeding strategy of BD, which is reflected in the ability to adjust the feeding preference according to nutritional needs and food environmental changes; thus, it is competitively advantageous and may partly explain why BD is becoming the predominant species and displays an extensive distribution in the ECS.
    Type of Medium: Online Resource
    ISSN: 2296-7745
    URL: Article
    URL: Article
    DOI: 10.3389/fmars.2021.728773
    DOI: 10.3389/fmars.2021.728773.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2757748-X
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  • 8
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    Figure 3 from: Liu C, Zhang J, Liu S, Song P, Guan Y, Shan B, Li Y, Lin L (2021) Genetic diversity of the yellowfin seabream, Acanthopagrus latus (Actinopterygii: Perciformes: Sparidae)—An enhancement species in Dongshan Bay. Acta Ichthyologica et Piscatoria 51(3): 281-287. https://doi.org/10.3897/aiep.51.66894
    Pensoft Publishers ; 2021
    In:  Acta Ichthyologica et Piscatoria Vol. 51, No. 3 ( 2021-09-09), p. 281-287
    Details
    In: Acta Ichthyologica et Piscatoria, Pensoft Publishers, Vol. 51, No. 3 ( 2021-09-09), p. 281-287
    Abstract: Dongshan Bay is located on the west side of the Taiwan Strait, which had abundant fishery resources in the past. With the increase in fishing pressure, resources have declined. To restore the fishery resources in Dongshan Bay and to increase fishing yield, much enhancement and release work has been carried out in Dongshan Bay. The yellowfin seabream, Acanthopagrus latus (Houttuyn, 1782), is an important enhancement species in Dongshan Bay that is also frequently captured. Due to yearly progress in enhancement and release, it is necessary to study the current status of the genetic diversity of yellowfin seabream in Dongshan Bay. The results show that all yellowfin seabream populations have high genetic diversity, which is mainly related to its breeding habits and growth rate, and this ensures a large recruitment stock in the natural seas. The current population has differentiated from the historical population due to a change in genetic structure, and many historical haplotypes have been lost. The results of this study provide a reference for fishery management departments to formulate management measures and conservation policies specifically for yellowfin seabream. In particular, yellowfin seabream is a hermaphroditic and protandrous species. Targeting an older age group as the main fishing subject is not conducive to its breeding protection and resource growth, and therefore, fishing of an older age group should be restricted in fishery production.
    Type of Medium: Online Resource
    ISSN: 1734-1515 , 0137-1592
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3897/aiep.51.66894
    DOI: 10.3897/aiep.51.66894.figure1
    DOI: 10.3897/aiep.51.66894.figure2
    DOI: 10.3897/aiep.51.66894.figure3
    Language: Unknown
    Publisher: Pensoft Publishers
    Publication Date: 2021
    detail.hit.zdb_id: 441372-6
    detail.hit.zdb_id: 2177242-3
    SSG: 21,3
    SSG: 12
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  • 9
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    Observation of the Y(4230) and a new structure in *
    IOP Publishing ; 2022
    In:  Chinese Physics C Vol. 46, No. 11 ( 2022-11-01), p. 111002-
    Details
    In: Chinese Physics C, IOP Publishing, Vol. 46, No. 11 ( 2022-11-01), p. 111002-
    Abstract: The cross sections of at center-of-mass energies from 4.127 to 4.600 GeV are measured based on 15.6 fb data collected with the BESIII detector operating at the BEPCII storage ring. Two resonant structures are observed in the line shape of the cross sections. The mass and width of the first structure are measured to be ( ) MeV and ( ) MeV, respectively. They are consistent with those of the established . The second structure is observed for the first time with a statistical significance greater than 8 σ , denoted as . Its mass and width are determined to be ( ) MeV and ( ) MeV, respectively. The first presented uncertainties are statistical and the second ones are systematic. The product of the electronic partial width with the decay branching fraction is reported.
    Type of Medium: Online Resource
    ISSN: 1674-1137 , 2058-6132
    URL: Article
    DOI: 10.1088/1674-1137/ac945c
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2022
    detail.hit.zdb_id: 2491278-5
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  • 10
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    Search for the weak decay *
    IOP Publishing ; 2023
    In:  Chinese Physics C Vol. 47, No. 1 ( 2023-01-01), p. 013002-
    Details
    In: Chinese Physics C, IOP Publishing, Vol. 47, No. 1 ( 2023-01-01), p. 013002-
    Abstract: Using events collected with the BESIII detector, we perform the first search for the weak baryonic decay . The analysis procedure is optimized using a blinded method. No significant signal is observed, and the upper limit on the branching fraction ( ) of is set as at the 90% confidence level.
    Type of Medium: Online Resource
    ISSN: 1674-1137 , 2058-6132
    URL: Article
    DOI: 10.1088/1674-1137/ac9895
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2023
    detail.hit.zdb_id: 2491278-5
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