In:
Molecular and experimental biology in medicine, Croatian Society of Biologists in Health Care, Vol. 2, No. 2 ( 2019-10-05), p. 51-56
Abstract:
Colony-forming unit
(CFU) assay is a short-term culture assay used for the detection of functionally active hematopoietic progenitor cells (HPCs) in the in vitro setting. This potency assay
enables the identification of colony-producing HPCs in any type of hematopoietic stem cell/progenitor product (HSC/P) including bone marrow (BM),
cord blood (CB) and mobilized peripheral blood (MPB). It has been shown that the frequency of HPCs in BM, MPB and CB cellular products directly correlates with the engraftment of both neutrophils and platelets as well as with the
overall survival of recipients following hematopoietic stem cell transplantation which makes the CFU assay a good quality parameter for the
prediction of engraftment success. The aim of this article is to provide an overview of different approaches to setting up the CFU assay with an emphasis
on different sample preparation techniques, cell plating density and colony identification and enumeration used by different laboratories.
Type of Medium:
Online Resource
ISSN:
2584-671X
Language:
Unknown
Publisher:
Croatian Society of Biologists in Health Care
Publication Date:
2019
Permalink
