In:
Frontiers in Fungal Biology, Frontiers Media SA, Vol. 2 ( 2022-1-28)
Abstract:
α-1,3-Glucan is one of the main polysaccharides in the cell wall of Aspergillus nidulans . We previously revealed that it plays a role in hyphal aggregation in liquid culture, and that its molecular mass (MM) in an agsA -overexpressing ( agsA OE ) strain was larger than that in an agsB -overexpressing ( agsB OE ) strain. The mechanism that regulates its MM is poorly understood. Although the gene amyD , which encodes glycosylphosphatidylinositol (GPI)-anchored α-amylase (AmyD), is involved in the biosynthesis of α-1,3-glucan in A . nidulans , how it regulates this biosynthesis remains unclear. Here we constructed strains with disrupted amyD (Δ amyD ) or overexpressed amyD ( amyD OE ) in the genetic background of the ABPU1 (wild-type), agsA OE , or agsB OE strain, and characterized the chemical structure of α-1,3-glucans in the cell wall of each strain, focusing on their MM. The MM of α-1,3-glucan from the agsB OE amyD OE strain was smaller than that in the parental agsB OE strain. In addition, the MM of α-1,3-glucan from the agsA OE Δ amyD strain was greater than that in the agsA OE strain. These results suggest that AmyD is involved in decreasing the MM of α-1,3-glucan. We also found that the C-terminal GPI-anchoring region is important for these functions.
Type of Medium:
Online Resource
ISSN:
2673-6128
DOI:
10.3389/ffunb.2021.821946
DOI:
10.3389/ffunb.2021.821946.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
3059082-6
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