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  • 1
    In: Ciência Rural, FapUNIFESP (SciELO), Vol. 46, No. 5 ( 2016-05), p. 901-908
    Abstract: RESUMO: Pesquisas têm sido realizadas para identificar as proteínas presentes no plasma seminal canino, com o intuito de relacioná-las com a qualidade espermática, bem como buscar por marcadores moleculares de patologias do trato reprodutivo. Há evidências de que as proteínas ligadoras de heparina, ligadoras de zinco, a lactoferrina, bem como as enzimas matrix metalloproteinase, superoxide dismutase, catalase e a glutationa peroxidase estão relacionadas com a qualidade seminal canina. Outras pesquisas indicam que a prolactina, e as enzimas arginina esterase, fosfatase ácida e fosfatase alcalina poderiam ser utilizadas com sucesso como biomarcadores de doenças reprodutivas. Assim, esta revisão de literatura objetiva abordar aspectos relacionados às proteínas do plasma seminal canino, suas influências sobre a fertilidade, e sua importância como biomarcadores de doenças reprodutivas.
    Type of Medium: Online Resource
    ISSN: 0103-8478
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2016
    detail.hit.zdb_id: 2025834-3
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  • 2
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 30, No. 6 ( 2001-12), p. 1704-1711
    Abstract: A study was conducted to determine the effect of heat stress on semen criteria and testis size in Santa Inês hairy rams, in the State of Ceará, Northeast of Brazil. The scrotum of eight adult rams was insulated for seven days. Animals were evaluated twice before insulation and, after insulation, at 12 different periods until 118 days. Scrotal insulation did not affect semen volume, but pH was increased after eight days, returning to normal values after 15 days. Sperm concentration was reduced eight days after insulation was removed and animals became azzospermic between 33 and 50 days. After 79 days, sperm concentration returned to values similar to those observed before treatment. Scrotal circumference (26.4 cm) decreased to 22.4 cm eight days after insulation and to 21 cm at 21 days, but returned to 24.9 cm after 50 days. Moreover, sperm motility and vigor were reduced right after insulation was removed and returned to normal profiles only after 90 days. Before treatment, there were 1.7% of sperm cells with primary defects and 9.9% with secondary defects. Right after insulation, sperm cells with primary defects increased to 3.6% and those with secondary defects, to 43.4%, and after eight days, these values increased to 8.4 and 60.4%, respectively. From 15 to 60 days, sperm cells with primary defects varied from 27.3 to 16.8%, while those with secondary defects showed only small variations (39.9 to 39%). At the end of the experiment (118 days after insulation), primary defects were reduced to 0.7%, but the percentage of cells with secondary defects was still high (24.4 %). Therefore, heat stress caused temporary interruption of sperm production in the ram and sperm motility and secondary defects seemed to be the most sensitive criteria.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2001
    detail.hit.zdb_id: 2078814-9
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  • 3
    Online Resource
    Online Resource
    Pontificia Universidade Catolica do Parana - PUCPR ; 2012
    In:  Revista Acadêmica Ciência Animal Vol. 10, No. 4 ( 2012-10-15), p. 335-
    In: Revista Acadêmica Ciência Animal, Pontificia Universidade Catolica do Parana - PUCPR, Vol. 10, No. 4 ( 2012-10-15), p. 335-
    Abstract: O estudo avaliou a tolerância ao calor de um rebanho de fêmeas leiteiras mestiças (Bos taurus x Bos indicus) criadas em região litorânea do estado do Ceará. Foram avaliados os parâmetros ambientais para o cálculo do índice de temperatura e umidade (ITU) e a temperatura retal (TR) para o cálculo do índice de tolerância ao calor (ITC) durante o período chuvoso e seco do ano. Observou-se que os valores da TR encontravam-se dentro da normalidade quando se avaliou os dados médios em ambos os períodos. Os valores médios de temperatura ambiente (TA) estavam superiores à zona de conforto térmico para animais taurinos, com resultados bem próximos do limite máximo para mestiços e zebuínos quando se analisou os dados de máxima no turno da tarde do período seco. Os valores de umidade relativa do ar (UR) mostraram um comportamento inverso à temperatura ambiente, observando-se sempre os maiores valores no período chuvoso, bem como no turno da manhã quando comparado com o turno da tarde, em ambos os períodos. Os valores encontrados para o ITU mostraram condições de ambiente que passaram de críticos para emergenciais quando não se avaliaram somente os dados médios. Ambos os índices de tolerância ao calor mostraram uma menor tolerância ao calor dos animais no período seco do ano. Conclui-se que os animais sofrem com o calor em ambos os períodos, sendo o período seco menos favorável, em que foram verificados valores mais elevados de TR, TA, ITU e menores de ITC.
    Type of Medium: Online Resource
    ISSN: 2596-2868
    Language: Unknown
    Publisher: Pontificia Universidade Catolica do Parana - PUCPR
    Publication Date: 2012
    detail.hit.zdb_id: 3007483-6
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  • 4
    Online Resource
    Online Resource
    FapUNIFESP (SciELO) ; 2006
    In:  Revista Brasileira de Zootecnia Vol. 35, No. 4 suppl ( 2006-08), p. 1629-1638
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 35, No. 4 suppl ( 2006-08), p. 1629-1638
    Abstract: This study aimed to evaluate changes in hormone secretion and in seminiferous epithelium of Angus bulls between 10 and 38 weeks of age. Samples of testicular parenchyma and blood were collected from 25 animals castrated in 4 week intervals. Traits associated to testicular development and quantitative aspects of spermatogenesis and hormonal concentrations were transformed by logarithm before analyses of variance. Changes in testis and seminiferous tubule diameter and testis weight were more pronounced after 26 weeks of age. The percentage of testicular parenchyma occupied by seminiferous tubules increased from 49.3 to 75.2% from 10 to 38 weeks. Most tubules ( 〉 90%) had only Sertoli cells at 10 and 14 weeks, but the number of tubules with gonocytes and A spermatogonia increased at 18 (13.8±1.7%) and 22 weeks (19±1%). Tubules with B and intermediate spermatogonia became predominant at 26 weeks (24.5±8.2%) and those with spermatocytes as the most advanced germ cell type were more evident at 30 weeks (42.3±9.9%). Round spermatids were detected at 26 weeks and at 38 weeks of age, 62.3±1.5% of all tubules had either elongate or mature spermatids. Variations in testis growth (specially testis weight) after 26 weeks were coincident with the establishment of meiosis in the seminiferous tubules, morphological alterations in nucleus and nucleolus of the Sertoli cells (indicators of Sertoli cell differentiation), lower levels of androstenedione and significant increases in testosterone and estradiol 17beta. Associations between testis development and concentrations of FSH and LH were less evident.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2006
    detail.hit.zdb_id: 2078814-9
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  • 5
    In: DESAFIOS - Revista Interdisciplinar da Universidade Federal do Tocantins, Universidade Federal do Tocantins, Vol. 6, No. 2 ( 2019-04-28), p. 3-9
    Abstract: A ausência da categoria asinina nas normas técnicas de avaliação seminal promove a necessidade de informações reprodutivas desta espécie. Objetivou-se descrever o perfil proteômico do plasma seminal de jumentos da raça Pêga (Equus asinus). Utilizaram-se seis animais púberes, com peso 239±32,6 Kg e circunferência escrotal de 36,8±6,9 cm, criados em mesma propriedade no estado do Tocantins, Brasil. Foi realizada eletroforese unidimensional, utilizando 12,5% de acrilamida e 30μg de proteína. As bandas foram descoradas e digeridas com tripsina para análise em espectrômetro de massa ESI-Q-TOF. Através de bioinformática, pelo banco de dados UniProtKB, as proteínas foram identificadas. Os termos da ontologia genética foram obtidos a partir do software STRAP®. A média da concentração proteica do plasma seminal foi 23,6±12,6 μg/μL. Foram detectadas pelo menos 26 bandas por animal (QuantityOne®). Um total de 19 bandas e 52 proteínas, com pesos entre 9,51 e 155,9 kDa, foram identificadas pela espectrometria. Os processos biológicos mais relevantes ligados às proteínas identificadas foram a regulação (24%) e processo celular (22%). As funções moleculares das proteínas foram descritas como ligação (42%) e atividade catalítica (31%). Em conclusão, a existência da descrição do padrão eletroforético destas proteínas plasmáticas seminais contribuirão com a construção de parâmetros para fertilidade.Palavras-chave: Proteômica, eletroforese, sêmen.
    Type of Medium: Online Resource
    ISSN: 2359-3652
    Language: Unknown
    Publisher: Universidade Federal do Tocantins
    Publication Date: 2019
    detail.hit.zdb_id: 2857021-2
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  • 6
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 32, No. 1 ( 2003-02), p. 240-248
    Abstract: A study was conducted to evaluate the use of cashew nuts meal (CNM) in diets for sheep, raised in feedlot. Thirty two crossbred animals received concentrates with the same amount of crude protein, but four different levels of CNM (0, 12, 24 and 36%). A factorial 4 x 2 was used, with four levels of CNM and 2 sexes, with four repetitions. Daily weight gain and feed conversion as well as intake of dry matter (DM), crude protein (CP), ether extract (EE), neutral detergent fiber(NDF) and ashes were determined by analysis of variance, Duncan statistical test and regression analysis. Intake was estimated as g/animal/day, percentage of body weight (%BW) and g/BW0.75. There were differences in intake of DM, CP and EE when animals were fed concentrates with 0 and 36% of CNM. Also, intake of DM, CP and NDF tended to decrease as the levels of CNM increased in the diet. EE intake increased according to the different CNM levels. The type of diet did not significantly affect either weight gain or feed conversion. Therefore, cashew nut meal can be included up to 24% in the concentrate, but the total diets should not have more than 6% of fat.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2003
    detail.hit.zdb_id: 2078814-9
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  • 7
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 30, No. 2 ( 2001-04), p. 360-367
    Abstract: A study was conducted to evaluate the effects of bST in crossbred dairy cows (Bos indicus x Bos taurus) raised in a semi-arid zone, in the Northeast of Brazil. Fifteen cows (second and third lactation; 42-155 days in milk) received five applications of bST, at 14-day intervals. Other 15 cows were used as controls. The animals were fed with diet consisted in elephant grass and concentrate. During the first application of bST, the milk production of the treated cows (12.6 kg/day) did not differ from the control animals (11.3 kg/day), but in the second and third applications, the difference between the groups was significant (2.0 and 2.3 kg). In the fourth application of bST, there was an increase in the difference between groups (3.0 kg) and in the fifth application, such difference was 3.4 kg (14.0 kg versus 10.6 kg/day). The bST treatment and stage of lactation interaction was not significant. Rectal and milk temperatures were higher (0.1 and 0.6 °C, respectively) in bST treated than in control animals, but differences were not significant. For the group of 30 cows, the daily milk yield was related to milk temperature (r = 0.38 to 0.69). Based on the temperature-humidity index, the cows were under light heat stress in the morning and moderate stress in the afternoon. Therefore, crossbred cows raised in the semi-arid area of Brazil showed responses to bST without apparent signs of severe heat stress.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2001
    detail.hit.zdb_id: 2078814-9
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  • 8
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 33, No. 3 ( 2004-06), p. 668-678
    Abstract: A study was conducted to determine the effect of environmental stress on physiological criteria of feedlot sheep. Treatments consisted of two different housing conditions (shade and no shade) and two levels of concentrate in the diet: high concentrate (70% of concentrate and 30% of roughage-70C:30R); low concentrate (30% of concentrate and 70% of roughage-30C:70R). Animals raised under shade and fed a high concentrate diet (70C:30R) had greater dry mater intake (1258 g/animal/day) and crude protein (0.8% of body weight (BW) and 18 g/BW0.75), as well as weight gain (247 g/animal/day). Air temperature in the afternoon was 30.6:C, under shade, and 32.1:C, under sun and values of temperature humidity index(THI), 82.3 and 81.1, respectively. Animals fed more concentrate (70C:30R) had also higher respiratory frequency (RF), either raised under shade (87 mov/min) or under the sun (71 mov/min.). Rectal temperature (RT) was higher in the afternoon (39.1:C) than in the morning (38.9:C), but animals raised under shade and fed diets with 70% concentrate (70C:30R) had the highest value of RT (39.3:C). Regardless of type of housing conditions, the animals that received more concentrate (70C:30R) had greater rectal temperature (39.2:C) than those fed a diet with less concentrate (30C:70R) (38.8:C). Therefore, the type of diet influenced the response of the animals to housing conditions. Moreover, Santa Inês sheep are susceptible to environmental stress because had lower performance when raised under no shade.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2004
    detail.hit.zdb_id: 2078814-9
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  • 9
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 34, No. 1 ( 2005-02), p. 54-59
    Abstract: The accumulated productivity (ACP) is an index that takes into account the total weight of calves weaned per cow (in kg), total time required for producing the calves and age at first calving. A study was conducted to estimate the components of genetic and residual variance and estimate the heritability of ACP index. Data set consisted of 2,816 observations of Nelore cows raised in different farms of the Brazilian North and Northeast. These farms participate in the Genetic Improvement Program of the Nelore Breed. The observed mean and respective standard deviation for ACP was 96.74 ± 46.70 kg of weaned calves/cow/year, with a coefficient of variation of 48.27%. This mean ACP, however, is lower than values estimated in other herds of the Nelore genetic program. Also, Standard Nelore cows showed higher ACP in comparison to cows of the Nelore Polled variety. Estimated heritability for ACP was 0.11 ± 0.06, suggesting that there is sufficient genetic variability that justify the use of ACP in genetic selection. However, results of such strategy depend, mainly, on efficient herd management.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2005
    detail.hit.zdb_id: 2078814-9
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  • 10
    In: Revista Brasileira de Zootecnia, FapUNIFESP (SciELO), Vol. 35, No. 3 suppl ( 2006-06), p. 988-996
    Abstract: The objective of this study was to estimate genetic and phenotypic parameters for reproductive traits of Nelore cows raised in the North and Northeast regions of Brazil. The traits studied were age at first calving (AFC), calving interval (CI), post-partum interval (PPI), number of services per conception (NSC) and gestation length (GL). The statistical analyses were done using the SAS program (Statistical Analysis System) and variance components were estimated by Restricted Maximum Likelihood method using the MTDFREML software. Means and heritability estimates for AFC, CI, PPI, NSC and GL were 45.14±10.83 months; 465.55±128.49 days; 165.76±110.29 days; 1.34±0.67 services and 295.03±5.85 days and 0.21±0.05; 0.05±0.02; 0.32±0.11; 0.05±0.04 e 0.12±0.04, respectively. The repeatability for CI, PPI, NSC and GL were, respectively, 0.05; 0.63; 0.09 and 0.22. The results suggest that despite the small genetic variability for the reproductive traits, AFC and GL must be included in selection programs. The high heritability estimate for PPI is probably associated to the structure of the data, in its majority coming from the same herd. The repeatability estimate for CI suggests that female culling based on first calving interval is not accurate and there is a risk of culling animals with probable good reproductive efficiency.
    Type of Medium: Online Resource
    ISSN: 1516-3598
    Language: Unknown
    Publisher: FapUNIFESP (SciELO)
    Publication Date: 2006
    detail.hit.zdb_id: 2078814-9
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