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  • 1
    Online Resource
    Online Resource
    RNA-Seq Analysis Provides the First Insights into the Phylogenetic Relationship and Interspecific Variation between Agropyron cristatum and Wheat
    Frontiers Media SA ; 2017
    In:  Frontiers in Plant Science Vol. 8 ( 2017-09-21)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 8 ( 2017-09-21)
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    DOI: 10.3389/fpls.2017.01644
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2017
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
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  • 2
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    Physical Mapping of a Novel Locus Conferring Leaf Rust Resistance on the Long Arm of Agropyron cristatum Chromosome 2P
    Frontiers Media SA ; 2018
    In:  Frontiers in Plant Science Vol. 9 ( 2018-6-18)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 9 ( 2018-6-18)
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    DOI: 10.3389/fpls.2018.00817
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2018
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
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  • 3
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    Table_1.xlsx
    Frontiers Media SA ; 2022
    In:  Frontiers in Oncology Vol. 12 ( 2022-8-29)
    Details
    In: Frontiers in Oncology, Frontiers Media SA, Vol. 12 ( 2022-8-29)
    Abstract: Glioblastoma (GBM) is a highly invasive neurological malignancy with poor prognosis. LncRNA-GAS5 (growth arrest-specific transcript 5) is a tumor suppressor involved in multiple cancers. In this study, we explored the clinical significance, biological function, and underlying mechanisms of GAS5 in GBM. We showed that lncRNA-GAS5 expression decreased in high-grade glioma tissues and cells, which might be associated with poor prognosis. GAS5 overexpression lowered cell viability, suppressed GBM cell migration and invasion, and impaired the stemness and proliferation of glioma stem cells (GSCs). We further discovered that GAS5 inhibited the viability of glioma cells through miR-let-7e and miR-125a by protecting SPACA6 from degradation. Moreover, GAS5 played an anti-oncogenic role in GBM through the combined involvement of let-7e and miR-125a in vivo and in vitro . Notably, these two miRNAs block the IL-6/STAT3 pathway in tumor tissues extracted from a xenograft model. Taken together, our study provides evidence for an important role of GAS5 in GBM by affecting the proliferation and migration of GSCs, thus providing a new potential prognostic biomarker and treatment strategy for GBM.
    Type of Medium: Online Resource
    ISSN: 2234-943X
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fonc.2022.803652
    DOI: 10.3389/fonc.2022.803652.s001
    DOI: 10.3389/fonc.2022.803652.s002
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2649216-7
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  • 4
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    Data_Sheet_1.docx
    Frontiers Media SA ; 2022
    In:  Frontiers in Plant Science Vol. 13 ( 2022-2-24)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-2-24)
    Abstract: As an important wheat wild relative, the P genome of Agropyron cristatum (L.) Gaertn. (2 n = 4 x = 28) is very valuable for wheat improvement. A complete set of wheat- A. cristatum disomic addition lines is the basis for studying the genetic behavior of alien homoeologous chromosomes and exploring and utilizing the excellent genes. In this study, a wheat- A. cristatum derivative II-11-1 was proven to contain a pair of 5P chromosomes and a pair of 2P chromosomes with 42 wheat chromosomes by analyzing the fluorescence in situ hybridization (FISH) and expressed sequence tag (EST) markers. Additionally, cytological identification and field investigation showed that the 5P chromosome can weaken the homologous pairing of wheat chromosomes and promote the pairing between homoeologous chromosomes. This provides new materials for studying the mechanism of the alien gene affecting the homologous chromosome pairing and promoting the homoeologous pairing of wheat. In addition, chromosomal structural variants have been identified in the progeny of II-11-1. Therefore, the novel 5P addition line might be used as an important genetic material to widen the genetic resources of wheat.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2022.844348
    DOI: 10.3389/fpls.2022.844348.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2687947-5
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  • 5
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    DataSheet_1.pdf
    Frontiers Media SA ; 2020
    In:  Frontiers in Plant Science Vol. 11 ( 2020-3-2)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 11 ( 2020-3-2)
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2020.00175
    DOI: 10.3389/fpls.2020.00175.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
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  • 6
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    Image_1.pdf
    Frontiers Media SA ; 2018
    In:  Frontiers in Plant Science Vol. 9 ( 2018-10-30)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 9 ( 2018-10-30)
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2018.01422
    DOI: 10.3389/fpls.2018.01422.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2018
    detail.hit.zdb_id: 2687947-5
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  • 7
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    Table_2.XLSX
    Frontiers Media SA ; 2022
    In:  Frontiers in Plant Science Vol. 13 ( 2022-5-12)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-5-12)
    Abstract: Rye ( Secale cereale L.), a naturally cross-pollinating relative of wheat, is a tertiary gene donor and of substantial value in wheat improvement. Wheat powdery mildew is caused by Blumeria graminis f. sp. tritici ( Bgt ), which seriously affects yield and quality worldwide. Identifying and transferring new, effective resistance genes against powdery mildew from rye is important for wheat breeding. The current study developed a wheat-rye line YT2 resistant to powdery mildew by crossing, backcrossing, and self-pollination for multiple generations between octoploid triticale 09R2-100 and common wheat cultivar Shixin 616. YT2 was confirmed to be a 6R disomic addition and T1RS⋅1BL translocation line by genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), multicolor-GISH (mc-GISH), and molecular marker analyses. Disease responses to different Bgt isolates and genetic analysis showed that the powdery mildew resistance gene of YT2 was derived from the rye chromosome 6R of 09R2-100, which differed from the previously reported Pm genes from rye including Pm20 on 6RL. Resistance phenotype of different translocation lines and deletion lines derived from YT2 combined with newly developed 6RL-specific markers analysis suggested that the powdery mildew resistance gene of YT2 was localized to the region in chromosome 6RL: 890.09–967.51 Mb and flanked by markers XM189 and X4M19 , corresponding to the reference genome of Weining rye. Therefore, YT2 could be used as a promising bridging parent for wheat disease resistance improvement.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2022.889494
    DOI: 10.3389/fpls.2022.889494.s001
    DOI: 10.3389/fpls.2022.889494.s002
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2687947-5
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  • 8
    Online Resource
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    Four-Year and Five-Developing-Stage Dynamic QTL Mapping for Tiller Number in the Hybrid Population of Agropyron Gaertn.
    Frontiers Media SA ; 2022
    In:  Frontiers in Plant Science Vol. 13 ( 2022-2-24)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-2-24)
    Abstract: Tiller number (TN) is an important agronomic trait affecting gramineous crop yield. To understand the static and dynamic information of quantitative trait locus (QTLs) controlling TN of Agropyron Gaertn., both the unconditional and conditional quantitative trait loci (QTL) mapping of TN were conducted using a cross-pollinated (CP) hybrid population with a total of 113 plant lines from the cross between Agropyron cristatum (L.) Gaertn. Z1842 and Allium mongolicum Keng Z2098, based on the phenotypic data of TN at five developmental stages [i.e., recovering stage (RS), jointing stage (JS), heading stage (HS), flowering stage (FS), and maturity stage (MS)] in 4 years (i.e., 2017, 2018, 2020, and 2021) and the genetic map constructed of 1,023 single-nucleotide polymorphism (SNP) markers. Thirty-seven QTLs controlling TN were detected using two analysis methods in 4 years, which were distributed in six linkage groups. Each QTL explained 2.96–31.11% of the phenotypic variation, with a logarithum of odds (LOD) value of 2.51–13.95. Nine of these loci detected both unconditional and conditional QTLs. Twelve unconditional major QTLs and sixteen conditional major QTLs were detected. Three relatively major stable conditional QTLs, namely, cQTN1-3 , cQTN1-5 , and cQTN4-1 , were expressed in 2020 and 2021. Meantime, two pairs of major QTLs cQTN1-5 and qTN1-4 and also cQTN2-4 and qTN2-3 were located at the same interval but in different years. Except for qTN2-2 and qTN3-5 / cQTN3-5 , other thirty-four QTLs were first detected in this study. This study provides a better interpretation of genetic factors that selectively control tiller at different developmental stages and a reference for molecular marker-assisted selection in the related plant improvement.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    DOI: 10.3389/fpls.2022.835437
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2687947-5
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  • 9
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    Image_2.tif
    Frontiers Media SA ; 2022
    In:  Frontiers in Plant Science Vol. 13 ( 2022-10-26)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-10-26)
    Abstract: Powdery mildew caused by Blumeria graminis f. sp. tritici ( Bgt ) is a destructive disease of wheat throughout the world. Host resistance is considered the most sustainable way to control this disease. Powdery mildew resistance gene Pm2b was mapped to the same genetic interval with Pm2a and PmCH1357 cloned previously, but showed different resistance spectra from them, indicating that they might be caused by different resistance genes or alleles. In this study, Pm2b was delimited to a 1.64 Mb physical interval using a large segregating population containing 4,354 F 2:3 families of resistant parent KM2939 and susceptible cultivar Shimai 15. In this interval, TraesCS5D03G0111700 encoding the coiled-coil nucleotide-binding site leucine-rich repeat protein (CC-NBS-LRR) was determined as the candidate gene of Pm2b . Silencing by barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) technology and two independent mutants analysis in KM2939 confirmed the candidate gene TraesCS5D03G0111700 was Pm2b . The sequence of Pm2b was consistent with Pm2a/PmCH1357. Subcellular localization showed Pm2b was located on the cell nucleus and plasma membrane. Pm2b had the highest expression level in leaves and was rapidly up-regulated after inoculating with Bgt isolate E09. The yeast two-hybrid (Y2H) and luciferase complementation imaging assays (LCI) showed that PM2b could self-associate through the NB domain. Notably, we identified PM2b interacting with the transcription factor TaWRKY76-D, which depended on the NB domain of PM2b and WRKY domain of TaWRKY76-D. TaWRKY76-D negatively regulated the resistance to powdery mildew in wheat. The specific KASP marker K529 could take the advantage of high-throughput and high-efficiency for detecting Pm2b and be useful in molecular marker assisted-selection breeding. In conclusion, cloning and disease resistance mechanism analysis of Pm2b provided an example to emphasize a need of the molecular isolation of resistance genes, which has implications in marker assisted wheat breeding.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2022.973065
    DOI: 10.3389/fpls.2022.973065.s001
    DOI: 10.3389/fpls.2022.973065.s002
    DOI: 10.3389/fpls.2022.973065.s003
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2687947-5
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  • 10
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    Image Recognition–Based Identification of Multifractal Features of Faults
    Frontiers Media SA ; 2022
    In:  Frontiers in Earth Science Vol. 10 ( 2022-5-17)
    Details
    In: Frontiers in Earth Science, Frontiers Media SA, Vol. 10 ( 2022-5-17)
    Abstract: Geologists have made several advances in applying multifractal theory in geology; however, some questions such as a large statistical workload and low efficiency remain unanswered. Thus, this study proposes an image recognition–based method for calculating fault multifractality. First, grayscale processing and binarization of the fault distribution map were performed. The image was then gridded, and the grids were numbered. Subsequently, computer image recognition technology was used to count the number of faults in each grid as a replacement for manual counting. Finally, the fractal dimensions of the faults were calculated using a multifractal box-counting algorithm. This method was successfully applied to fracture studies of the Maokou Formation in southeast Sichuan. Compared to the conventional approach, the proposed method demonstrated considerably improved work efficiency and accuracy. The results showed that the faults in the study area exhibited good statistical self-similarity in the scale range, indicating fractal characteristics. The fractal dimensions of faults with different orientations and the planar distribution of the fractal dimension contours indicate tectonic stages and stress magnitude in the study area. The results indicate that the tectonic setting of southeast Sichuan was formed primarily during the Indosinian, Yanshanian, and Himalayan periods. From the Indosinian to the early Yanshanian periods, NE-trending faults with relatively large fractal dimensions developed under NW–SE compressional tectonic stress. From the Late Yanshanian to Early Himalayan, EW-trending faults were formed by relatively weak N–S compressional stress and had the lowest fractal dimensions. The NW-trending faults formed by intense NE–SW compressional tectonic stress in the Late Himalayan region had the highest fractal dimensions. To promote oil and gas migration and ensure that faults do not destroy the caprock, oil and gas reservoirs must be in a relatively mild tectonic environment. Thus, the fractal dimensions of faults in favorable areas should be neither too high nor too low. The relationship between the fractal dimensions of faults and well test results in southeast Sichuan indicates that the region along the wells “ls1–xia14–guan3” (with fractal dimensions of 1.49–1.57) in the study area is a relatively favorable region for oil and gas preservation.
    Type of Medium: Online Resource
    ISSN: 2296-6463
    URL: Article
    DOI: 10.3389/feart.2022.909166
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2741235-0
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