In:
Acta Parasitologica, Springer Science and Business Media LLC, Vol. 63, No. 2 ( 2018-06-26), p. 280-286
Abstract:
The present study was designed to determine and analyze the mt genomes of Metastrongylus salmi ( M . salmi ), and reveal the phylogenetic relationships of this parasite using mt DNA sequences. Results showed that the complete mt genome of M . salmi was 13722 bp containing 12 protein-coding genes (cox1-3, nad1-6, nad4L, atp6 and cytb), 22 transfer RNA genes, and 2 ribosomal RNA genes (rrnL and rrnS). The overall A+T content was 73.54% and the nucleotide composition was A (23.52%), C (6.14%), G (19.60%), T (50.02%), and N (UCAG) (0.73%). A total of 4237 amino acids are encoded from the Tibetan isolates of M. salmi mt genomes. The ATA was predicted as the most common starting codon with 41.7% (5/12 protein genes); and 11 of the 12 protein genes were found to have a TAG or TAA translation termination codon. By clustering together the phylogenetic trees of Tibetan M . salmi and Austrian M . salmi , the M . salmi isolated from Tibetan pigs was found to be highly homological with that stemmed from Austrian one. This information provides meaningful insights into the phylogenetic position of the M . salmi China isolate and represents a useful resource for selecting molecular markers for diagnosis and population studies.
Type of Medium:
Online Resource
ISSN:
1896-1851
,
1230-2821
DOI:
10.1515/ap-2018-0032
Language:
Unknown
Publisher:
Springer Science and Business Media LLC
Publication Date:
2018
detail.hit.zdb_id:
2235366-5
SSG:
12
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