In:
Biological Chemistry, Walter de Gruyter GmbH, Vol. 382, No. 3 ( 2001-03-21), p. 355-361
Abstract:
A statistical method for the analysis of fluorescence fluctuation amplitudes including bright spikes is presented. This situation arises e. g. when fluorescent ligands interact with receptors carrying multiple binding sites. The technique gives information on the amount of bound ligand in solution, making it a complementary technique to fluorescence correlation spectroscopy analysis, which cannot be applied in this situation. Two simple statistical tests are proposed that can discriminate between fluorescence intensities originating from free ligands or complexes. The performance of the two tests is evaluated and compared on mixtures of a fluorophore and fluorophore coated beads that mimic the behaviour of multiliganded complexes. An application to ligand binding to the serotonin receptor, expressed on Escherichia coli cells, is also provided. Specific binding of a fluorophore to this receptor, as well as competition with several ligands, is assessed.
Type of Medium:
Online Resource
ISSN:
1431-6730
Language:
Unknown
Publisher:
Walter de Gruyter GmbH
Publication Date:
2001
detail.hit.zdb_id:
1466062-3
SSG:
12
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