In:
Frontiers in Cellular and Infection Microbiology, Frontiers Media SA, Vol. 13 ( 2023-6-14)
Kurzfassung:
Atopic dermatitis (AD) is a chronic inflammatory skin condition whose pathogenesis involves genetic predisposition, epidermal barrier dysfunction, alterations in the immune responses and microbial dysbiosis. Clinical studies have shown a link between Staphylococcus aureus and the pathogenesis of AD, although the origins and genetic diversity of S. aureus colonizing patients with AD is poorly understood. The aim of the study was to investigate if specific clones might be associated with the disease. Methods WGS analyses were performed on 38 S. aureus strains, deriving from AD patients and healthy carriers. Genotypes (i.e. MLST, spa- , agr- and SCC mec -typing), genomic content (e.g. virulome and resistome), and the pan-genome structure of strains have been investigated. Phenotypic analyses were performed to determine the antibiotic susceptibility, the biofilm production and the invasiveness within the investigated S. aureus population. Results Strains isolated from AD patients revealed a high degree of genetic heterogeneity and a shared set of virulence factors and antimicrobial resistance genes, suggesting that no genotype and genomic content are uniquely associated with AD. The same strains were characterized by a lower variability in terms of gene content, indicating that the inflammatory conditions could exert a selective pressure leading to the optimization of the gene repertoire. Furthermore, genes related to specific mechanisms, like post-translational modification, protein turnover and chaperones as well as intracellular trafficking, secretion and vesicular transport, were significantly more enriched in AD strains. Phenotypic analysis revealed that all of our AD strains were strong or moderate biofilm producers, while less than half showed invasive capabilities. Conclusions We conclude that in AD skin, the functional role played by S. aureus may depend on differential gene expression patterns and/or on post-translational modification mechanisms rather than being associated with peculiar genetic features.
Materialart:
Online-Ressource
ISSN:
2235-2988
DOI:
10.3389/fcimb.2023.1194254
DOI:
10.3389/fcimb.2023.1194254.s001
DOI:
10.3389/fcimb.2023.1194254.s002
DOI:
10.3389/fcimb.2023.1194254.s003
DOI:
10.3389/fcimb.2023.1194254.s004
DOI:
10.3389/fcimb.2023.1194254.s005
DOI:
10.3389/fcimb.2023.1194254.s006
DOI:
10.3389/fcimb.2023.1194254.s007
DOI:
10.3389/fcimb.2023.1194254.s008
DOI:
10.3389/fcimb.2023.1194254.s009
DOI:
10.3389/fcimb.2023.1194254.s010
DOI:
10.3389/fcimb.2023.1194254.s011
Sprache:
Unbekannt
Verlag:
Frontiers Media SA
Publikationsdatum:
2023
ZDB Id:
2619676-1
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