In:
Frontiers in Neuroinformatics, Frontiers Media SA, Vol. 17 ( 2023-3-8)
Abstract:
The rapid effects of estradiol on membrane receptors are in the focus of the estradiol research field, however, the molecular mechanisms of these non-classical estradiol actions are poorly understood. Since the lateral diffusion of membrane receptors is an important indicator of their function, a deeper understanding of the underlying mechanisms of non-classical estradiol actions can be achieved by investigating receptor dynamics. Diffusion coefficient is a crucial and widely used parameter to characterize the movement of receptors in the cell membrane. The aim of this study was to investigate the differences between maximum likelihood-based estimation (MLE) and mean square displacement (MSD) based calculation of diffusion coefficients. In this work we applied both MSD and MLE to calculate diffusion coefficients. Single particle trajectories were extracted from simulation as well as from α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor tracking in live estradiol-treated differentiated PC12 (dPC12) cells. The comparison of the obtained diffusion coefficients revealed the superiority of MLE over the generally used MSD analysis. Our results suggest the use of the MLE of diffusion coefficients because as it has a better performance, especially for large localization errors or slow receptor movements.
Type of Medium:
Online Resource
ISSN:
1662-5196
DOI:
10.3389/fninf.2023.1005936
DOI:
10.3389/fninf.2023.1005936.s001
DOI:
10.3389/fninf.2023.1005936.s002
DOI:
10.3389/fninf.2023.1005936.s003
DOI:
10.3389/fninf.2023.1005936.s004
DOI:
10.3389/fninf.2023.1005936.s005
DOI:
10.3389/fninf.2023.1005936.s006
DOI:
10.3389/fninf.2023.1005936.s007
DOI:
10.3389/fninf.2023.1005936.s008
DOI:
10.3389/fninf.2023.1005936.s009
DOI:
10.3389/fninf.2023.1005936.s010
DOI:
10.3389/fninf.2023.1005936.s011
DOI:
10.3389/fninf.2023.1005936.s012
DOI:
10.3389/fninf.2023.1005936.s013
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2452979-5
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