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  • Frontiers Media SA  (3)
  • Unknown  (3)
  • 1
    Online Resource
    Online Resource
    DataSheet_1.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Plant Science Vol. 14 ( 2023-3-28)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 14 ( 2023-3-28)
    Abstract: Hemsleya chinensis is a Chinese traditional medicinal plant, containing cucurbitacin IIa (CuIIa) and cucurbitacin IIb (CuIIb), both of which have a wide range of pharmacological effects, including antiallergic, anti-inflammatory, and anticancer properties. However, few studies have been explored on the key enzymes that are involved in cucurbitacins biosynthesis in H. chinensis . Oxidosqualene cyclase (OSC) is a vital enzyme for cyclizing 2,3-oxidosqualene and its analogues. Here, a gene encoding the oxidosqualene cyclase of H. chinensis ( HcOSC6 ), catalyzing to produce cucurbitadienol, was used as a template of mutagenesis. With the assistance of AlphaFold2 and molecular docking, we have proposed for the first time to our knowledge the 3D structure of HcOSC6 and its binding features to 2,3-oxidosqualene. Mutagenesis experiments on HcOSC6 generated seventeen different single-point mutants, showing that single-residue changes could affect its activity. Three key amino acid residues of HcOSC6, E246, M261 and D490, were identified as a prominent role in controlling cyclization ability. Our findings not only comprehensively characterize three key residues that are potentially useful for producing cucurbitacins, but also provide insights into the significant role they could play in metabolic engineering.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2023.1138893
    DOI: 10.3389/fpls.2023.1138893.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2613694-6
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  • 2
    Online Resource
    Online Resource
    Highly Efficient Dual-Beam Frequency Scanning Based on SSPPs With Parasitic Patch Array
    Frontiers Media SA ; 2022
    In:  Frontiers in Physics Vol. 10 ( 2022-2-17)
    Details
    In: Frontiers in Physics, Frontiers Media SA, Vol. 10 ( 2022-2-17)
    Abstract: A leaky-wave antenna (LWA) realizes radiation of travelling wave along guiding structures. Periodic modulation is an effective approach to turn non-radiating modes into radiating modes. A high-efficient LWA based on spoof surface plasmon polaritons (SSPPs) loaded with parasitic patch array is proposed. Sinusoidal modulation is used for the radiation of SSPPs, while parasitic array mitigates the open stopband (OSB) effect. A prototype is fabricated and measured. The measured and simulated results agree well with each other. Such design shows wide angle coverage of 83 ° from backward to forward and stable gain distribution of about 12 dB within the whole operating band.
    Type of Medium: Online Resource
    ISSN: 2296-424X
    URL: Article
    DOI: 10.3389/fphy.2022.851121
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2721033-9
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  • 3
    Online Resource
    Online Resource
    DataSheet_1.pdf
    Frontiers Media SA ; 2024
    In:  Frontiers in Immunology Vol. 15 ( 2024-7-19)
    Details
    In: Frontiers in Immunology, Frontiers Media SA, Vol. 15 ( 2024-7-19)
    Abstract: The type I interferon (IFN) pathway is important for eukaryotic cells to resist viral infection, as well as an impediment to efficient virus replication. Therefore, this study aims to create an IFNAR1 knockout (KO) Madin-Darby bovine kidney (MDBK) cell line using CRISPR/Cas9 and investigate its application and potential mechanism in increasing viral replication of bovines. The IFNAR1 KO cells showed increased titers of bovine viral diarrhea virus (BVDV) (1.5 log10), with bovine enterovirus and bovine parainfluenza virus type 3 (0.5–0.8 log10). RNA-seq revealed reduced expression of the genes related IFN-I pathways including IFNAR1, STAT3, IRF9, and SOCS3 in IFNAR1 KO cells compared with WT cells. In WT cells, 306 differentially expressed genes (DEGs) were identified between BVDV-infected and -uninfected cells. Of these, 128 up- and 178 down-regulated genes were mainly associated with growth cycle and biosynthesis, respectively. In IFNAR1 KO cells, 286 DEGs were identified, with 82 up-regulated genes were associated with signaling pathways, and 204 down-regulated genes. Further, 92 DEGs were overlapped between WT and IFNAR1 KO cells including ESM1, IL13RA2, and SLC25A34. Unique DEGs in WT cells were related to inflammation and immune regulation, whereas those unique in IFNAR1 KO cells involved in cell cycle regulation through pathways such as MAPK. Knocking down SLC25A34 and IL13RA2 in IFNAR1 KO cells increased BVDV replication by 0.3 log10 and 0.4 log10, respectively. Additionally, we constructed an IFNAR1/IFNAR2 double-knockout MDBK cell line, which further increased BVDV viral titers compared with IFNAR1 KO cells (0.6 log10). Overall, the IFNAR1 KO MDBK cell line can support better replication of bovine viruses and therefore provides a valuable tool for bovine virus research on viral pathogenesis and host innate immune response.
    Type of Medium: Online Resource
    ISSN: 1664-3224
    URL: Article
    URL: Article
    DOI: 10.3389/fimmu.2024.1404649
    DOI: 10.3389/fimmu.2024.1404649.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2024
    detail.hit.zdb_id: 2606827-8
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