In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-5-13)
Abstract:
Chlamydomonas reinhardtii has emerged as a powerful green cell factory for metabolic engineering of sustainable products created from the photosynthetic lifestyle of this microalga. Advances in nuclear genome modification and transgene expression are allowing robust engineering strategies to be demonstrated in this host. However, commonly used lab strains are not equipped with features to enable their broader implementation in non-sterile conditions and high-cell density concepts. Here, we used combinatorial chloroplast and nuclear genome engineering to augment the metabolism of the C. reinhardtii strain UVM4 with publicly available genetic tools to enable the use of inorganic phosphite and nitrate as sole sources of phosphorous and nitrogen, respectively. We present recipes to create phosphite-buffered media solutions that enable high cell density algal cultivation. We then combined previously reported engineering strategies to produce the heterologous sesquiterpenoid patchoulol to high titers from our engineered green cell factories and show these products are possible to produce in non-sterile conditions. Our work presents a straightforward means to generate C. reinhardtii strains for broader application in bio-processes for the sustainable generation of products from green microalgae.
Type of Medium:
Online Resource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2022.885840
DOI:
10.3389/fmicb.2022.885840.s001
DOI:
10.3389/fmicb.2022.885840.s002
DOI:
10.3389/fmicb.2022.885840.s003
DOI:
10.3389/fmicb.2022.885840.s004
DOI:
10.3389/fmicb.2022.885840.s005
DOI:
10.3389/fmicb.2022.885840.s006
DOI:
10.3389/fmicb.2022.885840.s007
DOI:
10.3389/fmicb.2022.885840.s008
DOI:
10.3389/fmicb.2022.885840.s009
DOI:
10.3389/fmicb.2022.885840.s010
DOI:
10.3389/fmicb.2022.885840.s011
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2587354-4
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