In:
Journal of Endocrinology, Bioscientifica, Vol. 194, No. 3 ( 2007-09), p. 529-537
Abstract:
We investigated effects of human ( h ) GH on the proliferation of h -hepatocytes that had been engrafted in the liver of albumin enhancer/promoter driven-urokinase plasminogen activator transgenic/severe combined immunodeficiency disease (uPA/SCID) mice (chimeric mice). The h -hepatocytes therein were considered to be deficient in GH, because h GH receptor ( h GHR) is unresponsive to mouse GH. Actually, h IGF-1 was undetectable in chimeric mouse sera. The uPA/SCID mice were transplanted with h -hepatocytes from a 6-year (6Y)-old donor, and were injected with recombinant h GH (r h GH). r h GH stimulated the repopulation speed of h -hepatocytes; and up-regulated h IGF-1, human signal transducers and activators of transcription ( h STAT) 3, and cell cycle regulatory genes such as human forkhead box M1, human cell division cycle 25A, and human cyclin D1. To confirm the reproducibility of these effects of r h GH, similar experiments were run using h -hepatocytes from a 46-year (46Y)-old donor. r h GH similarly enhanced their repopulation speed and up-regulated the expression of the above-tested genes, especially h IGF-1 and h STAT1. The extent of the enhancement by r h GH was much less than that in 6Y-hepatocyte-chimeric mice most probably due to the difference in GHR expression levels between the two donors. In conclusion, this study clearly demonstrated that r h GH stimulates the proliferation of h -hepatocytes in vivo .
Type of Medium:
Online Resource
ISSN:
0022-0795
,
1479-6805
Language:
Unknown
Publisher:
Bioscientifica
Publication Date:
2007
detail.hit.zdb_id:
1474892-7
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