In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-5-6)
Abstract:
Clubroot is caused by Plasmodiophora brassicae , which threatens Brassicaceae crop production worldwide. In recent years, there has been an outbreak and rapid spread of clubroot in many major cruciferous crop-producing areas of China. In this study, we identified a cabbage material DingWen (DW) with different resistant capabilities from Huashuang5R (H5R) and Huayouza62R of Brassica napus , which are currently used as the main resistant cultivars for clubroot management in China. We used a next-generation sequencing-based bulked segregant analysis approach, combined with genetic mapping to identify clubroot-resistant (CR) genes from F 1 population generated from a cross between the DW (CR) and HZSX (clubroot susceptible). The CR locus of DW (named CRA8.1 ) was mapped to a region between markers A08-4346 and A08-4853, which contains two different loci CRA8.1a and CRA8.1b after fine mapping. The CRA8.1b loci contain a fragment of 395 kb between markers A08-4624 and A08-4853 on A08 chromosome, and it is responsible for the resistance to PbZj and PbXm isolates. However, together with CRA8.1a , corresponding to a 765-kb region between markers A08-4346 and A08-4624, then it can confer resistance to PbXm + . Finally, through expression analysis between resistant and susceptible materials, two genes encoding TIR-NBS-LRR proteins ( BraA08g039211E and BraA08g039212E ) and one gene encoding an RLP protein ( BraA08g039193E ) were identified to be the most likely CR candidates for the peculiar resistance in DW.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.898108
DOI:
10.3389/fpls.2022.898108.s001
DOI:
10.3389/fpls.2022.898108.s002
DOI:
10.3389/fpls.2022.898108.s003
DOI:
10.3389/fpls.2022.898108.s004
DOI:
10.3389/fpls.2022.898108.s005
DOI:
10.3389/fpls.2022.898108.s006
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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