In:
Anti-Cancer Agents in Medicinal Chemistry, Bentham Science Publishers Ltd., Vol. 23, No. 12 ( 2023-07-10), p. 1457-1468
Abstract:
Prostate cancer is the second-leading cause of cancer death in men. Sinularin is a soft coralsderived
natural compound that has anticancer activity in many cancer cells. However, the pharmacological action of sinularin in prostate cancer is unclear. Aim: The aim of the study is to examine the anticancer effects of sinularin in prostate cancer cells. Methods: We explored the anticancer effects of sinularin on the prostate cancer cell lines, PC3, DU145, and LNCaP,
by MTT, Transwell assay, wound healing, flow cytometry, and western blotting. Results: Sinularin inhibited the cell viability and colony formation of these cancer cells. Furthermore, sinularin inhibited
testosterone-induced cell growth in LNCaP cells by downregulating the protein expression levels of androgen receptor (AR), type II 5α-reductase, and prostate-specific antigen (PSA). Sinularin significantly attenuated the invasion and
migration ability of PC3 and DU145 cells, with or without TGF-β1 treatment. Sinularin inhibited epithelialmesenchymal transition (EMT) in DU145 cells after 48 h of treatment by regulating the protein expression levels of Ecadherin,
N-cadherin, and vimentin. Sinularin induced apoptosis, autophagy, and ferroptosis by regulating the protein expression levels of Beclin-1, LC3B, NRF2, GPX4, PARP, caspase-3, caspase-7, caspase-9, cleaved-PARP, Bcl-2, and
Bax. Moreover, intracellular reactive oxygen species (ROS) were increased but glutathione was decreased after sinularin treatment in PC3, DU145 and LNCaP cells. Conclusion: Sinularin regulated the androgen receptor signaling pathway and triggered apoptosis, autophagy, and
ferroptosis in prostate cancer cells. In conclusion, the results indicated that sinularin may be a candidate agent for human prostate cancer and need further study for being applied to human.
Type of Medium:
Online Resource
ISSN:
1871-5206
DOI:
10.2174/1871520623666230331083744
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2023
SSG:
15,3
Permalink