In:
Frontiers in Pharmacology, Frontiers Media SA, Vol. 12 ( 2021-12-6)
Abstract:
Autoimmune hepatitis (AIH) is a chronic liver disease caused by disruption of liver immune homeostasis. The effect of dendritic cells (DCs) on the pathogenesis of AIH is not fully understood. Long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs) have been shown to play critical roles in the regulation of cell function. In this study, we analyzed the immunophenotypic characteristics of DCs in the peripheral blood. The percentage of mature DCs was higher in AIH patients than in healthy controls (HCs), and the proportion of mature DCs decreased after treatment. We isolated monocyte-derived DCs (moDCs) from the peripheral blood, obtained whole RNA-sequencing (RNA-seq) data for the moDCs from the two groups, and identified differentially expressed (DE) lncRNAs, circRNAs, miRNAs and mRNAs. In addition, we performed Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for the DE mRNAs and constructed competing endogenous RNA (ceRNA) networks. ENST00000543334, hsa_circ_0000279, and hsa_circ_0005076 were selected and validated by RT-qPCR. These results provide a possible molecular mechanism of DCs in the pathogenesis of AIH and identify some potential therapeutic targets.
Type of Medium:
Online Resource
ISSN:
1663-9812
DOI:
10.3389/fphar.2021.792138
DOI:
10.3389/fphar.2021.792138.s001
DOI:
10.3389/fphar.2021.792138.s002
DOI:
10.3389/fphar.2021.792138.s003
DOI:
10.3389/fphar.2021.792138.s004
DOI:
10.3389/fphar.2021.792138.s005
DOI:
10.3389/fphar.2021.792138.s006
DOI:
10.3389/fphar.2021.792138.s007
DOI:
10.3389/fphar.2021.792138.s008
DOI:
10.3389/fphar.2021.792138.s009
DOI:
10.3389/fphar.2021.792138.s010
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2021
detail.hit.zdb_id:
2587355-6
SSG:
15,3
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