In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 58, No. 7 ( 2010-02-18), p. 909-915
Abstract:
The object of this study was to compare the effect of chitosans of different number-average molecular weights (MWs) and degrees of acetylation (FA) on transepithelial transport of morphine in Caco-2 cells. Caco-2 monolayers on polycarbonate (PC) membranes (0.5cm2) were incubated with morphine (10μ) or mannitol (55μ) for 180 min. Samples for analysis of morphine (LCMSMS) and mannitol (liquid scintillation) were drawn at 45, 90, 120 and 180 min. Transepithelial electrical resistance (TEER) and transmission electron microscopy were used to monitor cell integrity. In controls, morphine transport was half that of mannitol. Chitosans affected the transport of morphine and mannitol similarly. For chitosans with similar FA (0.32-0.43) and varying MWs (7–200 kD), transport was increased at MWs of 29 kD or more. Among chitosans of similar MWs (180–300 kD) and varying FA (0.01-0.61), those with the highest Fa (0.61) had the least effect, while chitosans with Fa/MW 0.01/250 and 0.17/300 promoted the greatest transport. An FA/MW of 0.32/200 and 0.43/170 induced a high and stable transport rate. Chitosans may enhance transepithelial transport of morphine by the same mechanism as for mannitol. Chitosans with FA of 0.3-0.4 and MW of approx. 200 kD seem favourable in this respect.
Type of Medium:
Online Resource
ISSN:
0022-3573
,
2042-7158
DOI:
10.1211/jpp.58.7.0005
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2010
detail.hit.zdb_id:
2041988-0
detail.hit.zdb_id:
2050532-2
SSG:
15,3
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