In:
Current Bioactive Compounds, Bentham Science Publishers Ltd., Vol. 19, No. 6 ( 2023-07)
Abstract:
A cellular model of oxidative stress induced by hydrogen peroxide in the
primary culture of myoblasts was obtained by in vitro experiments, and the possibility of exogenous regulation of the cytotoxic effect using 2-ethyl-6-methyl-3-hydroxypyridine malate (ethoxidol)
was studied. Moreover, the in fluence of oxidative stress and the effect of ethoxidol on the
intracellular expression of such an important biomarker as myostatin was investigated. Methods: Hydrogen peroxide was used to induce oxidative stress. The effect of hydrogen peroxide
on the rate of myoblast proliferation was studied by measuring the reduction level of (3-(4,5- dimethylthiazole-2-yl))-2,5-diphenyltetrazolium bromide. To measure the expression of myostatin,
a real-time polymerase chain reaction (PCR-RV) method was used. Results: During the work, it was clearly demonstrated that hydrogen peroxide has a significant
cytostatic effect on myoblasts in vitro, inhibiting their proliferation. Ethoxidol in physiological concentration did not show toxic effects and did not inhibit cell proliferation. This antioxidant revealed
a statistically significant protective effect on the cytostatic effect of hydrogen peroxide on myoblasts. In addition, this compound inhibited the expression of myostatin mRNA caused by
exposure to hydrogen peroxide as a negative regulator of growth and differentiation of muscle tissue that occurs in response to exposure to reactive oxygen species. Conclusion: Hydrogen peroxide is one of the highly active forms of oxygen and has a significant
cytostatic effect on myoblasts in vitro, suppressing their proliferation. 2-ethyl-6-methyl-3- hydroxypyridine malate neutralizes the toxic effect of peroxide, thereby indirectly having a positive
effect on the rate of myoblast proliferation in vitro.
Type of Medium:
Online Resource
ISSN:
1573-4072
DOI:
10.2174/1573407219666221122122346
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2023
SSG:
15,3
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